Biomolecules

YMC Co.,Ltd.'s Wide-Pore Columns are reversed-phase products available with 300Å or 200Å pore size. A choice of functional groups from ODS, C8, C4, and CN are also available, enabling the analyst to choose the proper pore size and stationary phase.

• Reversed-phase column with wide pore size
• Packing materials with pore size of 300Å or 200Å are available (For AQ,200Å only)
• Useful for separation of proteins or peptides
• Useful for fast analysis of compounds with high hydrophobicity

The chromatograms show a comparison of the retention behavior of alkylbenzenes on both ODS-A(300Å) and ODS-A(120Å). The compounds with relatively high hydropohbicity can be eluted in a shorter retention time on ODS-A(300Å) than ODS-A(120Å)

Retention change per methylene group of ODS-A(300Å) is similar to that of ODS-A(120Å), indicating that alkyl chain density on the stationary phase is similar for these two columns. Since the slope of the line for ODS-A(300Å) is different from that for YMC-Pack C8,ODS-A(300Å) may show different separation behavior with regard to selectivity, despite having similar hydrophobicity. Thus, ODS-A(300Å), ODS-A(120Å) or C8 should be selected with care for rapid optimization of compound separation.

The graph shows the test result of the stability of the stationary phase with 0.1% aqueous TFA. Retention of diphenyl on C4 columns manufactured by other companies greatly decreases as time passes. This is caused by detachment of butyl groups from the packing material due to acid hydrolysis. Retention of diphenyl on YMC-Pack PROTEIN-RP is shown to be stable after 500 hours of mobile phase flow.