YMC-BioPro IEX Columns are for proteins and peptides, based on a newly developed hydrophilic polymer with low nonspecific absorption.
YMC-BioPro IEX Columns are available in QA and SP chemistries on 5 µm porous (QA and SP columns) and non-porous (QA-F and SP-F columns) hydrophilic polymer beads packed in biocompatible PEEK column housings in a variety of dimensions (inquire for details).
Please note that the same QA and SP chemistries are also available on 75 µm porous polymer beads supplied in bulk - See separate datasheet.
|YMC-BioPro QA||YMC-BioPro QA-F||YMC-BioPro SP||YMC-BioPro SP-F|
|Matrix||Hydrophilic Polymer Bead|
|Pore size||1000 Å||Non-porous||1000 Å||Non-porous|
|Counter ion||Cl -||Na +|
|0.075 - 0.100
|0.075 - 0.110
|0.070 - 0.095
|0.230 - 0.290
|> 110 mg
|> 12 mg
|> 70 mg
human IgG/mL resin
|> 10 mg
human IgG/mL resin
|pH Range||2.0 - 12.0|
For information and specifications covering BioPro 75 µm materials,
see separate datasheet.
- QA and SP chemistry
- Hydrophilic polymer bead
- Porous and Non-porous types
- Low operating pressure
- Excellent resolution
- High recovery of biomolecules
- Ultra-fast analysis on non-porous type
1. Ribonuclease Å (0.1 mg/mL)
|YMC-BioPro SP-F can separate the proteins sharply without peak-tailing observed on Brand T. Furthermore, despite larger particle size, the theoretical plate number of YMC-BioPro is higher than that of Brand T.|
Comparison of dynamic binding capacity (DBC) and recovery
|Dynamic binding capacity
|YMC-BioPro QA (5 µm, 50 x 4.6 mml.D.)||125||120||95|
|Brand G (10 µm, 50 x 5.0 mml.D.)||100||35||35|
|Brand T (10 µm, 50 x 4.6 mml.D.)||73||58||79|
* Recovery: (Eluted amount / Dynamic binding capacity) x 100
Compared with convential porous-polymer anion-exchange column, YMC-BioPro QA scores the superior DBC and recovery rate. Thus YMC-BioPro shows the least nonspecific absorption in conventional columns.
Comparison of the effect of sample load on YMC-BioPro QA and commercial Q type product
|YMC-BioPro QA shows the excellent peak shapes even when the loading amount increases. By contrast, the column of Brand G cannot separate well even in small amounts of injection.|