Normal Phase

YMC-Pack Polyamine II is a silica-based packing bonded with polyamine. It is particularly useful for separation of sugars. The column life of YMC-Pack Polyamine II in aqueous mobile phase is longer than conventional silica-based amino columns, and thus is applicable to separation of oligosaccharides using mobile phase with relatively higher water content. In addition, YMC-Pack Polyamine II can be used to separate ionic compounds with a combination of normal-phase mode and weak anion exchange mode.

• Silica gel chemically bonded to polyamine
• The most suitable column for separation of sugars
• Solves durability problem of conventional silica-based amino columns
• Useful for separation of hydrophilic compounds including vitamins
• Useful for separation of fat-soluble compounds using nonaqueous mobile phase

Flow conditions Column : YMC-Pack Polyamine II 250 x 4.6mmI.D. Eluent : acetonitrile/water Flow Rate : 1.0mL/min Temperature : ambient Time : 100 hours Analytical conditions Eluent : acetonitrile/water(75/25) Flow Rate : 1.0mL/min Temperature : 26°c Time : RI, 32 x 10-6 RIU/FS

Even in a hard flow durability test using mobile phase that consists only of water,the retention time of sugars changes little.

This figure shows differences in selectivity of phenols when separated on Diol-NP and SIL under the same conditions. For these compounds, Diol columns provide longer retention time and better separation.

1. Fructose 2. Glucose 3. Sucrose 4. Maltose 5 .Lactose Column : YMC-Pack Polyamine II, 250 x 4.6mmI.D. Eluent : acetonitrile/water (75/25) Flow Rate : 1.0mL/min Temperature : 26°c Time : RI, 32 x 10-6 RIU/FS

Column : YMC-Pack Polyamine II 250 x 4.6mmI.D. Eluent : n-hexane/ethyl acetate (70/30) Flow Rate : 1.0mL/min Temperature : 26°c Time : UV at 295 nm	Column : YMC-Pack Polyamine II 250 x 4.6mmI.D. Eluent : 50mM NH4H2PO4 /acetonitrile (30/70) Flow Rate : 1.0mL/min Temperature : 26°c Time : UV at 250 nm

YMC-Pack Polyamine II is applicable for separation of fat-soluble vitamins and water-soluble vitamins as a normal-phase column that can be used with water or buffer and various organic solvents.