Other Reversed Phase

Unique Selectivity

Phenyl columns provide unique selectivity when compared to aliphatic straight chain reversed phases such as ODS, C8 and C4. The π electrons of the phenyl group can interact with the aromatic ring of an analyte to increase retention relative to non-aromatic species. Phenyl also has application as an alternative to ODS and C4, for the analysis of peptides and proteins, on both small pore 120Å and wide-pore 300Å silica supports. Retention is decreased on wide-pore phenyl phases relative to 120Å phenyl material. Generally, as pore size increases surface area decreases, resulting in shorter retention times.

• Monomeric phenyl, fully endcapped
• Preferential retention of aromatic compounds
• Alternative to ODS or C4 bonded phases for the analysis or peptides and other biomolecules

1. p-Aminobenzoic acid (PABA) 2. Biotin (Vitamin H) 3. Riboflavin (Vitamin B2) 4. Cyanocobalamin (Vitamin B12) Column : 150 x 4.6mmI.D. Eluent : acetonitrile/50mM NH4H2PO4 (10/90) Flow Rate : 1.0 mL/min Temperature : 37°c Detection : UV at 254 nm Injection : 10µL (0.02-0.30mg/mL)

This figure shows difference in retention behavior of Ph and ODS for separation of water-soluble vitamins.