Other Reversed Phase
|Usable pH range||:||2.0-7.5|
Phenyl columns provide unique selectivity when compared to aliphatic straight chain reversed phases such as ODS, C8 and C4. The π electrons of the phenyl group can interact with the aromatic ring of an analyte to increase retention relative to non-aromatic species. Phenyl also has application as an alternative to ODS and C4, for the analysis of peptides and proteins, on both small pore 120Å and wide-pore 300Å silica supports. Retention is decreased on wide-pore phenyl phases relative to 120Å phenyl material. Generally, as pore size increases surface area decreases, resulting in shorter retention times.
- Monomeric phenyl, fully endcapped
- Preferential retention of aromatic compounds
- Alternative to ODS or C4 bonded phases for the analysis or peptides and other biomolecules
1. p-Aminobenzoic acid (PABA)
This figure shows difference in retention behavior of Ph and ODS for separation of water-soluble vitamins.