Other Reversed Phase

All phases in the Pro-family are ideal for the analysis of all types of organic molecules but specifically basic and metal chelating compounds. The ultrapure silica and very low residual silanol activity means that these columns frequently do not require the use of ion-pairing reagents or ion-suppression techniques, making them ideal for LC/MS applications.

• Consistently reproducible quality
• Documentation of each manufacturing step
• Consistently high packing quality and column efficiency
• Stringent specifications
• Individual test of each column (test chromatogram)
• Batch identification for each column

	1. Scoporamine bromide 2. Atropine sulfate 3. Cinchonin 4. Quinine 5. Papaverine chloride Column : YMC-Pack Pro C8 (5µm, 120Å) 150 x 4.6mmI.D. Eluent : acetonitrile/20mM KH2PO4 (20/80) Flow Rate : 1.0mL/min Temperature : 30°c Detection : UV at 210 nm

Effectively endcapped Pro C8 and C4 offer better peak shape for separation of basic compounds such as alkaloids.

Optimization of separation by Pro C8 and C4 1. Caffeine 2. Ethenzamide 3. Bromovalerylurea 4. Ibuprofen Column : 150 x 4.6mmI.D. Eluent : methanol/20mM H3PO4 (60/40) Flow Rate : 1.0mL/min Temperature : 37°c Detection : UV at 254 nm

This figure shows that Pro C8 and C4 columns allow us to minimize total analysis time while maintaining excellent separation for caffeine and ethenzamide and bromovalerylurea.

In the case that it is difficult to optimize resolution and analysis time on separation by C18, C8 and C4 columns can be effective alternatives.