Reversed Phase (ODS)

High Efficiency

The 4µm, 80Å (80Å) silica support provides
high chromatographic efficiency, while the
very high surface area material (500 m2/g)
offers exceptional interactions. This combination provides excellent peak symmetry.

Choose Your Retention

The J'sphere™ series combines three different carbon loads-9%, 14% and 22%-with a range of hydrogen-bonding capacities to provide three columns that differ in retention and often in selectivity as well. You can exploit these differences in method development without ever leaving of product and support chromatographers have come to expect from YMC.

ODS-H80, with 22% carbon is the most hydrophobic and hydrolytically stable. It resists attack by both high- and low-pH solvents and has proven stable at pH's 1.1 to 9.3 for over 500 hours!

ODS-M80, with 14% carbon, is an inert, efficient phase with moderate hydrophobicity and hydrogen bonding capacity.

ODS-L80, with 9% carbon offers retention similar to C8, but with unique selectivity imparted by its high hydrogen- bonding capacity.

These three columns afford the chromatogapher a range of retention on a pure, high-efficiency, reproducible support.

• Average over 100,000 plates / meter
• Inert, reproducible support
• Choice of three levels of hydrophobicity
• Excellent peak symmetry with basic molecules

Column : 150 X 4.6mmI.D. Eluent : acetonitrile/water (75/25) Flow Rate : 1.0 mL/min Temperature : 37°c Detection : UV at 254 nm	Column : 150X4.6mmI.D. Eluent : acetonitrile /20mM KH2PO4 (15/85) Flow Rate : 1.0 mL/min Temperature : 37°c Detection : UV at 254 nm

Three different types of ODS with different ligand coverage display different hydrophobicity and hydrogen bonding capacities and steric selectivity. ODS-H80 has higher hydrophobicity and less hydrogen bonding capacity. ODS-M80 has moderate hydrophobicity and hydrogen bonding capacity. ODS-L80 has less hydrophobicity similar to a C8 and high hydrogen-bonding capacity.

Under acid/alkaline conditions

Column : J'sphere ODS-H80 150 x 4.6 mmI.D. Eluent : acetonitrile / water (60/40) Flow Rate : 1.0 mL/min Temperature : 37°c Detection : UV at 270 nm Sample : butyl benzoate	Column : J'sphere ODS-H80 150 x 4.6 mmI.D. Eluent : acetonitrile / water (60/40) Flow Rate : 1.0 mL/min Temperature : 37°c Detection : UV at 270 nm Sample : butyl benzoate

ODS-H80 shows acid resistance and alkali resistance. It is effective for separation of peptides and basic compounds.