Reversed Phase (ODS)
|Usable pH range||:||2.0-7.5|
Successful use in highly aqueous eluents
ODS-AQ is a unique reversed-phase material with both a hydrophobic high carbon loading and a relatively hydrophilic surface. Because of the hydrophilic surface, ODS-AQ can be "wetted" with polar eluents. ODS-AQ provides chromatographers reproducible retention and excellent peak shape in highly aqueous eluents even 100% water. That is difficult with conventional ODS packings.
Strong Retention for Polar Compounds
In highly aqueous mobile phases, ODA-AQ gives longer retention than conventional ODS packings. This is because the C18 ligands on ODS-AQ are actually lifted from the surface by the action of the eluent penetrating to the silica pores.
Different Selectivity versus Typical ODS
Selectivity on ODS-AQ can be subtly different from that seen on conventional ODS columns. Differences in selectivity are most often encountered when analyzing mixtures of relatively polar compounds. Typically, longer retention and greater resolution are observed with ODS-AQ.
Suited for Biochemical Applications
ODS-AQ is well suited for analysis and purification of peptides and other biomolecules. Available in both 120Å and 200Å pore sizes, ODS-AQ is appropriate for all types of peptide analysis regardless of size.
- Recommended for analysis of highly polar compounds
- Eliminates need for paired ion reagents
- Strong retention in aqueous eluents
- Enhanced separation of biomolecules such as peptides, proteins and nucleotides
- Different selectivity versus conventional ODS
ODS-AQ is successfully applied under high aqueous mobile phase conditions such as for the separation of saccharides or glycoside.