Special
In general, reliable applications in HPLC require a minimum retention factor (k’) of 2. In the widely used reversed phase methods polar compounds such as peptides, organic acids or pyrimidines often exhibit little or no retention and therefore low resolution on a conventional C18 phase. The use of additives such as ion-pair reagents is not an alternative since it is not applicable to LC-MS. Also normal phase chromatography fails due to poor solubility of the polar compounds in organic solvents. HILIC fills the gap between reversed phase and normal phase chromatography, because polar compounds can be retained while using e.g. acetonitrile/water eluents and buffers suitable for LC-MS.
- YMC HILIC provides an ideal mechanism for the analysis of extremely polar analytes
- YMC offers a wide variety of HILIC phases to cover a broad range of polar selectivities
- HILIC eluents are compatible with LC-MS systems
- Nucloesides and bases
- Acrylamide
- Melamine in powdered cheese
- Melamine in dairy products: HILIC versus RP
- Reproducibility of YMC HILIC
- Comparison of YMC HILIC Diol with other conventional HILIC phases
- Comparison of YMC HILIC Diol with other conventional HILIC phases
| YMC-Pack Diol-NP 5 μm, 2.0 x 150 mm |
ZIC-HILIC 5 μm, 2.1 x 150 mm |
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| XBridge HILIC 5 μm, 2.1 x 150 mm |
LUNA HILIC 5 μm, 3.0 x 150 mm |
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1. Uracil 2. Uridine 3. Adenosine 4. Cytosine 5. Cytidine 6. Guanosine |
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Since the detection of the genotoxic compound, acrylamide in starchy foodstuffs subjected to prolonged heating, the analysis of this compound has been of major interest.
| Column: | YMC-Pack Diol-NP (5μm, 12nm) 150 x 2.0 mm ID |
| Eluent: | 20 mM CH3COONH4/ acetonitrile (5/95) |
| Flow rate: | 0.2 ml/min |
| Temperature: | 25°C |
| Detection: | UV at 210 nm |
| Injection: | 1 μl (40 μg/ml) |
| Column: | YMC-Pack Diol-NP (5μm, 12nm) 150 x 3.0 mm ID |
| Eluent: | 10 mM CH3COONH4/ methanol (5/95) |
| Flow rate: | 0.4 ml/min |
| Detection: | UV at 210 nm |
| Injection: | 5 μl |
| Temperature: | 40°C |
YMC-Pack Diol-NP (5 μm, 12 nm) 150 x 2.0 mm ID |
YMC-UltraHT Hydrosphere C18 (2 μm, 12 nm) 50 x 2.0 mm ID |
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| A) Powdered cheese (blank) B) Melamine-spiked powdered cheese (10 mg/kg; final 0.5 μg/ml) C) Standard (0.5 μg/ml) |
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| Column: | YMC-Pack Diol-NP (5μm, 12nm) 150 x 3.0 mm ID |
| Eluent: | 10 mM CH3COONH4/ methanol (5/95) |
| Flow rate: | 0.2 ml/min |
| Detection: | UV at 210 nm |
| Temperature: | 40°C |
| Sample: | melamine-spiked powdered cheese 0.5 μg/ml) |
| YMC-Pack Diol-NP 5 μm, 2.0 x 150 mm |
ZIC-HILIC 5 μm, 2.1 x 150 mm |
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| A) Powdered cheese (blank) B) Melamine-spiked powdered cheese (10 mg/kg; final 0.5 μg/ml) C) Standard (0.5 μg/ml) |
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| XBridge HILIC 5 μm, 2.1 x 150 mm |
LUNA HILIC 5 μm, 3.0 x 150 mm |
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| A) Powdered cheese (blank) B) Melamine-spiked powdered cheese (10 mg/kg; final 0.5 μg/ml) C) Standard (0.5 μg/ml) |
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| Eluent: | 10 mM CH3COONH4/ methanol (5/95) |
| Flow rate: | 0.2 ml/min (Diol-NP) 0.22 ml/min (ZIC-HILIC, XBridge) 0.45 ml/min (LUNA) |
| Temperature: | 40°C |
| Detection: | UV at 210 nm |
| YMC-Pack Diol-NP 5 μm, 2.0 x 150 mm |
ZIC-HILIC 5 μm, 2.1 x 150 mm |
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| XBridge HILIC 5 μm, 2.1 x 150 mm |
LUNA HILIC 5 μm, 3.0 x 150 mm |
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| 1. Cyanuric acid 2. Ammelide 3. Ammeline 4. Melamine |
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