Columns/YMC*Gel

YMC-BioPro IEX Columns

YMC-BioPro IEX Columns are for proteins and peptides, based on a newly developed hydrophilic polymer with low nonspecific absorption.

YMC-BioPro IEX Columns are available in QA and SP chemistries on 5 µm porous (QA and SP columns) and non-porous (QA-F and SP-F columns) hydrophilic polymer beads packed in biocompatible PEEK column housings in a variety of dimensions (inquire for details).

Please note that the same QA and SP chemistries are also available on 75 µm porous polymer beads supplied in bulk - See separate datasheet.

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Specifications, 5 µm analytical particles

	YMC-BioPro QA	YMC-BioPro QA-F	YMC-BioPro SP	YMC-BioPro SP-F
Matrix	Hydrophilic Polymer Bead
Particle size	5µm
Pore size	1000 Å	Non-porous	1000 Å	Non-porous
Charged group	-CH₂N⁺(CH₃)₃		-CH₂CH₂CH₂SO₃^-
Counter ion	Cl^-		Na⁺
Ion-exchange capacity	0.075 - 0.100 meq/mL resin	0.075 - 0.110 meq/mL resin	0.070 - 0.095 meq/mL resin	0.230 - 0.290 meq/mL resin
Dynamic binding capacity	> 110 mg BSA/mL resin	> 12 mg BSA/mL resin	> 70 mg human IgG/mL resin	> 10 mg human IgG/mL resin
pH Range	2.0 - 12.0
Column Housing Material	PEEK

For information and specifications covering BioPro 75 µm materials,
see separate datasheet.

Features:

QA and SP chemistry
Hydrophilic polymer bead
Porous and Non-porous types
Low operating pressure

Benefits:

Excellent resolution
High recovery of biomolecules
Ultra-fast analysis on non-porous type

Resolution: comparison of theoretical plate number

1. Ribonuclease Å (0.1 mg/mL)
2. Cytochrome c (0.1 mg/mL)
3. Lysozyme (0.1 mg/mL)

Eluent	:	A) 20mM KH2PO4K2HPO4 (pH 6.8) B) 20mM KH2PO4K2HPO4 (pH 6.8) containing 0.5M NaCl
Gradient	:	YMC-BioPro SP-F 0-100 %B (0-4 min) Brand T 0-100 %B (0-4.67 min)
Flow rate	:	1.5 mL/min
Temperature	:	25 °C
Detection	:	UV at 220 nm
Injection	:	20 µL

YMC-BioPro SP-F can separate the proteins sharply without peak-tailing observed on Brand T. Furthermore, despite larger particle size, the theoretical plate number of YMC-BioPro is higher than that of Brand T.

Binding capacity and Recovery

Comparison of dynamic binding capacity (DBC) and recovery

	Dynamic binding capacity (mg/mL-gel, 10% breakthrough)	Eluted amount (mg/mL-gel)	Recovery* (%)
YMC-BioPro QA (5 µm, 50 x 4.6 mml.D.)	125	120	95
Brand G (10 µm, 50 x 5.0 mml.D.)	100	35	35
Brand T (10 µm, 50 x 4.6 mml.D.)	73	58	79

* Recovery: (Eluted amount / Dynamic binding capacity) x 100

Compared with convential porous-polymer anion-exchange column, YMC-BioPro QA scores the superior DBC and recovery rate. Thus YMC-BioPro shows the least nonspecific absorption in conventional columns.

Loadability

Comparison of the effect of sample load on YMC-BioPro QA and commercial Q type product

Eluent	:	A) 20mM Tris-HCl (pH 8.1) B) 20mM Tris-HCl (pH 8.1) containing 0.5M NaCl 10-90%B (0-30 min)
Flow rate	:	0.5 mL/min
Temperature	:	25 °C
Detection	:	UV at 280 nm
Injection	:	100 µL

YMC-BioPro QA shows the excellent peak shapes even when the loading amount increases. By contrast, the column of Brand G cannot separate well even in small amounts of injection.

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