Columns/YMC*Gel

YMC-BioPro IEX Columns are for proteins and peptides, based on a newly developed hydrophilic polymer with low nonspecific absorption.

Features:

• QA and SP chemistry
• Hydrophilic polymer bead
• Porous and Non-porous types
• Low operating pressure

Benefits:

• Excellent resolution
• High recovery of biomolecules
• Ultra-fast analysis on non-porous type

	1.  Ribonuclease Å (0.1 mg/mL) 2.  Cytochrome c (0.1 mg/mL) 3.  Lysozyme (0.1 mg/mL) Eluent : A) 20mM KH2PO4K2HPO4 (pH 6.8) B) 20mM KH2PO4K2HPO4 (pH 6.8) containing 0.5M NaCl Gradient : YMC-BioPro SP-F   0-100 %B (0-4 min) Brand T   0-100 %B (0-4.67 min) Flow rate : 1.5 mL/min Temperature : 25 °C Detection : UV at 220 nm Injection : 20 µL
YMC-BioPro SP-F can separate the proteins sharply without peak-tailing observed on Brand T. Furthermore, despite larger particle size, the theoretical plate number of YMC-BioPro is higher than that of Brand T.

Comparison of dynamic binding capacity (DBC) and recovery

* Recovery: (Eluted amount / Dynamic binding capacity) x 100

Compared with convential porous-polymer anion-exchange column, YMC-BioPro QA scores the superior DBC and recovery rate. Thus YMC-BioPro shows the least nonspecific absorption in conventional columns.

Comparison of the effect of sample load on YMC-BioPro QA and commercial Q type product

Eluent : A) 20mM Tris-HCl (pH 8.1) B) 20mM Tris-HCl (pH 8.1) containing 0.5M NaCl 10-90%B (0-30 min) Flow rate : 0.5 mL/min Temperature : 25 °C Detection : UV at 280 nm Injection : 100 µL	YMC-BioPro QA shows the excellent peak shapes even when the loading amount increases. By contrast, the column of Brand G cannot separate well even in small amounts of injection.