Columns/YMC*Gel

YMC CHIRAL CD BR series is composed of 3 types of optical isomer separation columns. Each column possesses α-,β-,or γ-bromo-cyclodextrin as a functional group. Selection from the 3 types of columns enables analysis of a wide range of compounds. In addition, YMC CHIRAL CD BR series shows different selectivity from ODS because the separation is based on host-guest interaction. YMC CHIRAL CD BR series is useful for separating structural isomers that are difficult to separate using ODS.

Column
YMC CHIRAL α-CD BR 250 x 4.6mmI.D.	YMC CHIRAL β-CD BR 250 x 4.6mmI.D.	YMC CHIRAL γ-CD BR 250 x 4.6mmI.D.
Eluent
0.1M CH3COOH-CH3COONa (pH4.0)/methanol(90/10)	methanol/water (20/80)	methanol/water (50/50)
Flow rate
1.0mL/min	1.0mL/min	1.0mL/min
25ºC	25ºC	30ºC
Detection
UV at 254 nm	UV at 254 nm	UV at 230 nm

α-CD BR,β-CD BR,γ-CD BR show different separation feature because each of them has different size of cavities. Therefore choosing proper one can result in providing a successful separation of a wide-range compounds. It is also effective to use together with ODS columns.

Column
YMC CHIRAL β-CD BR 250 x 4.6mmI.D.	YMC CHIRAL β-CD BR 250 x 4.6mmI.D.	YMC CHIRAL γ-CD BR 250 x 4.6mmI.D.
Eluent
0.5M triethylamine- acetic acid (pH4.0)/ acetonitrile(10/90)	0.5M triethylamine- acetic acid (pH5.4)/ acetonitrile(95/5)	0.5M triethylamine- acetic acid (pH4.0)/ acetonitrile(60/40)
Flow rate
1.0mL/min	1.0mL/min	1.0mL/min
Temperature
25ºC	25ºC	25ºC
Detection
UV at 254 nm	UV at 254 nm	UV at 230 nm

YMC CHIRAL CD BR series provide better separation for various optical isomers because cyclodextrin is a chiral compound. YMC CHIRAL CD BR series is effective as a chiral column for reversed phase eluent.