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A Stability-Indicating Method for Separation of Charge Variant Impurities of a PEGylated Protein by CEX-HPLC

Poster by Athena Papasodero, Christopher Warner
Ligand Pharmaceuticals, Pelican Expression Technology™ Group,
Analytical Development Team, San Diego, CA 92121, USA

In this novel method, use of the YMC BioPro SF column with UV detection significantly improved the separation of charge variants in PEGylated proteins that were produced in Pseudomonas fluorescens, the foundational basis of the Pelican Expression Technology™ platform.

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