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YMC-BioPro: Prep Overview

Overview

Overview

  • Sulfobutyl chemistry for cation (SCX) exchange
  • Quaternary amino chemistry for anion (SAX) exchange
  • Excellent dynamic binding capacity (DBC)
  • Low non-specific adsorption and high recovery
  • High capacity with low operating pressure even at high flow rates

YMC-BioPro Q30, Q75, S30, and S75 are high performance ion exchange resins designed for the purification of proteins, peptides, antibodies and oligonucleotides in laboratory and industrial scale.

They are based on a next generation hydrophilic polymer matrix with a particle size of either 30 µm or 75 µm, and a pore size of 100 nm.

On special request, YMC BioPro is also available with a particle size of 10 µm and a pore size of 100 nm. Please inquire for additional information.

The materials offer a high dynamic binding capacity, together with low non-specific adsorption and excellent recovery. YMC-BioPro has narrow particle size distribution to help sharpen resolution, and it exhibits a low operating pressure, making it easily applied in high flow applications.

The 30 µm materials are well-suited for purification of large sample volumes at high flow rates, whereas the 75 µm mateirlas are preferred for high capacity capture steps and efficient intermediate purification steps.

YMC-BioPro Preparative Screening Kits

Easy to use screening kits enable resin screening, purification method development, and loadability studies. These low cost kits make it easy to test combinations of conditions and materials. See Datasheet. YMC-BioPro materials are also available in Atoll* formats (please inquire).

pH Stability

pH Stability

YMC-BioPro can be used in the range of pH 2 – 12, enabling the use of a broader range of separation conditions.

Applications

Applications

Separation of human serum

YMC-BioPro Q75: separation of human serum

Column: YMC-BioPro Q75, 50 x 4.6 mm I.D.
Eluent : A) 20 mM Tris-HCI (pH 8.6)
B) 1.0 M NaCl in 20 mM Tris-HCI (pH 8.6)
Gradient: 0% B (0-3 min), 0-15% B (3-18 min),
15-50% B (18-33 min), 50% B (33-40 min)
Flow rate: 0.5 mL/min (180 cm/hr)
Temperature: 25ºC
Detection: UV at 280 nm
Injection: 20 mL (110 µL/mL)

Performance

Performance

Excellent efficiency with high dynamic binding capacity

BioPro Q75 and S75 give both the high capacity required for capture steps and the high efficiency needed for intermediate purification. This increases productivity and reduces costs in bioprocesses.

Specification

Anion
Exchanger
Particle Size
(µm)
Ion Exchange Capacity
(meq/mLresin)
DBC1)
(mg/mLresin)
YMC-BioPro Q75 75 0.13 187
Cation
Exchanger
Particle Size
(µm)
Ion Exchange Capacity
(meq/mLresin)
DBC1)
(mg/mLresin)
YMC-BioPro S75 75 0.12 187

1) Dynamic binding capacities were determined at 10% breakthrough under the following conditions:

For YMC-BioPro Q75 For YMC-BioPro S75
Column: 50 x 4.6 mm I.D. 50 x 4.6 mm I.D.
Linear velocity: 3.0 cm/min (180 cm/h) 3.0 cm/min (180 cm/h)
Detection: UV at 280 nm UV at 300 nm
Equilibration buffer: 20 mM Tris-HCI (pH 8.6) 20 mM Glycine-NaOH (pH 9.0)
Elution buffer: 0.5 M NaCl
in equilibration buffer
0.5 M NaCl
in equilibration buffer
Sample: 1.5 mg/ml BSA
in equilibration buffer
1.5 mg/ml Lysozyme
in equilibration buffer

 

YMC-BioPro Preparative Screening Kits

Easy to use screening kits enable resin screening, purification method development, and loadability studies. These low cost kits make it easy to test combinations of conditions and materials. See Datasheet. YMC-BioPro materials are also available in Atoll* formats (please inquire).

Availability

Availability

YMC-BioPro Q30, S30, Q75, and S75 are available in bulk quantities from 50 mL to several thousand litres. The materials is manufactured in large batches and long term supply is assured to accommodate industrial bioprocessing requirements.

To assist with process validation, regulatory support files and technical support documentation are available.

Ordering Information

Product Particle Size Code Packing Sizes
50 mL 250 mL 1 L 5 L 25 L*
YMC-BioPro Q30 30µm QAA0S30
YMC-BioPro S30 30µm SPA0S30
Product Particle Size Code Packing Sizes
50 mL 250 mL 1 L 5 L 25 L*
YMC-BioPro Q75 75µm QAA0S75
YMC-BioPro S75 75µm SPA0S75

* Larger or customized packing sizes are available on request.

Specifications

Specifications

YMC-BioPro Q30 YMC-BioPro S30
Matrix hydrophilic polymer beads
Particle Size 30 µm
Charged Group -CH2N+(CH3)3 -(CH2)4SO3
Dynamic Binding Capacity
(mg/mL-resin)
> 160 (BSA) > 160 (Lysozyme)
Ion Exchange Capacity
(meq/mL-resin)
> 0.1 > 0.1
pH range 2.0 – 12.0
YMC-BioPro Q75 YMC-BioPro S75
Matrix
Particle Size 30 µm
Charged Group -CH2N+(CH3)3 -(CH2)4SO3
Dynamic Binding Capacity
(mg/mL-resin)
> 160 (BSA) > 160 (Lysozyme)
Ion Exchange Capacity
(meq/mL-resin)
> 0.1 > 0.1
pH range 2.0 – 12.0

Dynamic binding capacities were determined at 10% breakthrough under the following conditions:

Column: 50 x 4.6 mm I.D.
Linear Velocity: 3.0 cm/min (180 cm/hr)
For Anion Exchange (Q Type)
Equilibration buffer: 20 mM Tris-HCI (pH 8.6)
Elution buffer: 0.5 M NaCl in equilibration buffer
Sample: 1.5 mg/mL BSA in equilibration buffer
Detection: UV at 280 nm
For Cation Exchange (S Type)
Equilibration buffer: 20 mM Glycine-NaOH (pH 9.0)
Elution buffer: 0.5 M NaCl in equilibration buffer
Sample: 1.5 mg/mLl Lysozyme in equilibration buffer
Detection: UV at 300 nm

Application/ Scale-Up

Scale-Up

YMC-BioPro series includes pre-packed columns with 5 µm porous and non-porous resins for fast analysis, and bulk materials of 30 µm and 75 µm porous polymer beads for purification and capture.

The 5 µm porous types of BioPro QA and SP have similar retention selectivity to the 30 µm and 75 µm BioPro bulk media and allow for predictable scale-up from laboratory to production scale.

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