Separate Mixed-Polarity Samples Quickly
YMC-Pack C4 (Butyl) columns are less hydrophobic than C8 or C18 packings, and generally utilize more aqueous eluents than either of these reversed-phase column types. When compared to C8 and C18 columns (using the same eluents), C4 stationary phases show significantly shorter retention for non-polar compounds. Retention of polar compounds, however, is not significantly affected. Therefore, mixtures with a wide range of component polarity are best analyzed by YMC-Pack C4. This is due to the C4 bonded phase exhibiting shorter analysis time while still maintaining high resolution when compared to longer-chain bonded chemistries.
YMC-Pack C4 (Butyl) columns are available with three pore sizes to accommodate optimum separations of virtually any molecular-weight compound. The 120Å phase is used extensively with non-polar peptides which are too strongly retained on C8 and C18. The 200Å and 300Å pore butyl phase columns effectively resolve many classes of proteins and biopolymers. The wide pore butyl chemistry allows minimal distortion of tertiary conformation for large biomolecules and results in samples that are pure, concentrated, and high in biological activity.
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