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YMC-Triart: Prep Overview



  • Scalable
  • pH resistant
  • Mechanically stable
  • Base-washable (clean in place)
  • Particle sizes up to 50 micron
  • Variety of phases for selectivity
  • Cost effective

YMC-Triart is a novel multi-layered hybrid material prepared in a well-controlled particle formation process adapted from YMC’s microreactor technology. The resultant particle is highly resilient to a wide range of pH, temperature, and mechanical stresses. These properties make columns packed from Triart particles extremely resistant to degradation that normally would cause premature failure of columns packed with silica based materials.

As a result, users of YMC-Triart materials are not subject to the usual constraints of mobile phase pH, separation temperature, or pressure limitations encountered in columns packed with silica based particles.

Important advantages of YMC-Triart in purification applications.

Base-washable — YMC-Triart materials are chemically resistant to low and high pH (C18 and C8 are stable from pH 1-12), so the material may be cleaned in place by reverse-flow using alkaline washing. This is an important alternative compared to the expense of replacing the silica based column material, and the lost time spent in emptying, cleaning and re-packing a column… not to mention excessive potential downstream process downtime.

Long Life — YMC-Triart particles are resistant to fracture when subjected to the rigors of axial compression; and are easily recovered, stored, and repacked without particle breakdown.

Familiar selectivities — YMC-Triart C18 and C8 phases are designed as higher performing replacements for typical ODS and Octyl phase selectivities.

New selectivities — YMC-Triart is also available in Phenyl, PentaFluoroPhenyl (PFP), and Diol HILIC phases, enabling the use of selectivity profiles different from those of C8 or C18.

Larger Particle Sizes — YMC-Triart Prep is offered in particle sizes of 10, 15, 20, and 50 micron – in lot sizes large enough to satisfy the requirements of large scale preparative chromatography.

Bulk Availability — YMC-Triart is the first hybrid material readily available in multi-kg bulk quantity. Plus, you can use YMC-Triart anywhere in the world, through any of our YMC-operated subsidiaries.

pH Stability

pH Stability

Wider range of separation conditions possible

YMC-Triart is stable across a pH range that is hostile to most other stationary phases. This gives chromatographers larger freedom to choose separation parameters most suitable for the target compound(s). The wide pH ranges for the various Triart phase types can be seen below:

Triart pH ranges

This enables purification with a mobile phase containing TFA and cleaning with alkaline solution, which are often used on peptides and proteins purification.  Some examples are:

Triart Prep chart

Rinsing, Washing, Cleaning-in-Place (CIP)

Because of its chemical stability, YMC-Triart materials can be subjected to aggressive washing steps using low or high concentrations of organic solvents, and using high pH alkali rinses – with outstanding success. In addition, because of the mechanical strength of YMC-Triart, these steps can be performed using flow-through and/or reverse flow.

Alkali wash

Results for a CIP cycle are shown above. After 20 injections of the stress test mixture the peak shape becomes distorted and the retention time decreases. After regeneration of the column with 0.1 M NaOH/acetonitrile (50/50) (3 column volumes), the retention behavior returned back to normal.

YMC-Triart 10 alkali CIP

As shown above, even after 10 alkali CIP/wash steps with 0.1M NaOH/acetonitrile, the separation reverts to its initial performance (within acceptable limits).

This extension in life time will not save not only the cost of bulk packing material, but the time involved for less frequent column packing. This gives a smoother process and contributes to a more cost efficient production process.

Mechanical Strength

Mechanical Strength

YMC-Triart is Physically Rugged

Beyond its chemical stability, in terms of mechanical/physical strength, when compared to typical spherical silica – including high strength silica – YMC-Triart is as strong or stronger.

These SEM photographs show unused material (all are 15µm particles with pore sizes as indicated). YMC-Triart Prep has good size uniformity and very little cracked material (no cracked material appears in this image of the YMC-Triart material). In addition, the smooth surface of YMC-Triart Prep enables uniform surface modification. These mechanical advantages contribute to easy and successful column packing.

Triart Prep
(15µm 20 nm)

Triart Prep
Competitor A
(15µm 20 nm) Daiso ODSRPS
Competitor B
(15µm 10 nm) Fuji Chromatorex ODS

Physically Resilient – YMC-Triart is mechanically stable under the rigors of wetting with organic solvents and aqueous buffers. YMC-Triart can withstand accelerated flow, increased pressure, and reversed flow.

Re-packing – The physical durability of YMC-Triart also makes it amenable to unpacking, storage, and re-packing. The physical stresses of un-packing a column can lead to shearing and crushing of the packing material. The fractured particles – fines – congest the interstitial spacing of the spherical particles, which leads to an increase in backpressure.

The test results below demonstrate the advantage – compared to conventional silica – of the mechanical strength of both YMC-HG-series materials and YMC-Triart materials… with YMC-Triart exhibiting superior particle performance and stability from the initial packing onward.

Repacking Text Diagram

Spherical C8 Comparison

Below are SEM images of YMC-Triart C8-s and another spherical C8 material of the same particle and pore size. Both are shown after the initial packing (n=1), and after ten packing-unpacking cycles (n=10). Fines are numerous in the conventional silica after 10 repackings.

SEM particle image


Elevated Temperature – While typically not needed, if desirable, YMC-Triart is also well-suited to use at elevated temperatures up to 50ºC (even higher for C18 and C8).

Physical Properties

Physical Properties

Proprietary Particle manufacturing Process

YMC-Triart is a novel multilayered hybrid material prepared in a well-controlled particle formation process adapted from YMC’s microreactor technology.

The resulting material exhibits remarkable uniformity in terms of particle size and pore size. This uniformity exists not only within the batch or lot, but also across different batches and lots.

YMC-Triart Pore Size Distribution

The chart above shows the pore size distribution typical to YMC-Triart Prep. Particle and pore size uniformity contribute to the outstanding peak shape and reproducibility of the YMC-Triart materials. In purification applications, peak shape is critical to fraction recovery and purity.

Particle Purity

The YMC-Triart particle itself is of very high purity, and virtually metal-free.


YMC-Triart analytical scale materials (5 micron and smaller) are end-capped using methodology suitable for analytical scale separations (C18 and C8 both employ multi-stage end-capping; Phenyl uses a single step process; PFP is not end-capped).

YMC-Triart prep scale materials (10 micron and larger) use a single step process for C18, C8, and Phenyl. Carbon loading is carefully managed to produce correlate-able results at any scale. PFP prep materials are not end-capped.



Bulk Pack

Available materials (further modifications possible, please inquire):

Product Number Pore size Particle Size Description Pack sizes
TAS12S11 12 nm 10 µm YMC-Triart Prep C18-5
TAS12S16 12 nm 15 µm YMC-Triart Prep C18-5
TAS12S50 12 nm 20 µm YMC-Triart Prep C18-5
TAS12S50 12 nm 50 µm YMC-Triart Prep C18-5
TOS20S11 20 nm 10 µm YMC-Triart Prep C8-5
TOS20S16 20 nm 15 µm YMC-Triart Prep C8-5
TOS20S50 20 nm 20 µm YMC-Triart Prep C8-5
TOS20S50 20 nm 50 µm YMC-Triart Prep C8-5

Pre-Packed Columns

YMC-Triart columns are offered in a wide variety of scales and configurations.

  • UHPLC columns
  • Narrow i.d. HPLC columns
  • HPLC columns
  • Semi-Prep Axial Compression columns (Actus)
  • Semi-Prep standard columns
  • Prep columns

Many of these configurations are described elsewhere in this website; please contact YMC America with questions about product availability.



Product Name YMC-Triart Prep C18-S YMC-Triart Prep C8-S
Particle Size (µm) 10 15 20 10 15 20
Pore Size (nm) 12 20
Carbon Content (%) 20 13
Bulk Density (g/mL) Approx. 0.58 Approx. 0.52
pH Range 2-10 for regular use (up to pH 12 for alkaline CIP)
Typical Theoretical Plate Number * (/m) >25,000 >16,000 >12,000 >23,000 >15,000 >11,000
Product Number TAS12S11 TAS12S16 TAS12S21 TOS20S11 TOS20S16 TOS20S21

Application/ Scale-Up

Application/ Scale-Up

Scale-up from Lab to Pilot to Plant

Separation selectivity of YMC-Triart Prep material (10, 15, 20, 50 µm) preparative C18-S is equivalent to analytical-scale Triart C18 (1.9, 3, 5 µm).

YMC-Triart C18 and YMC-Triart Prep C18-S

  1. Oxytocin (MW 1,007)
  2. Met-Enkephalin (MW 574)
  3. Leu-Enkephalin (MW 556)
  4. Neurotensin (MW 1,673)
  5. γ-Endorphin (MW 1,859)
  6. β-Endorphin (MW 3,465)
Column: 150 x 4.6 mm I.D.
Eluent : A) water/TFA (100/0.1)
B) acetonitrile/TFA (100/0.1)
20-40% B (0-20 min)
Flow rate: 1.0 mL/min
Temperature: 37ºC
Detection: UV at 220 nm
Injection: 10 µL (0.167 mg/mL)

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