Reversed Phase (Octyl)

YMC-Triart C8 is a novel, multi-layered, organic/inorganic hybrid particle synthesized with C8 (L7 USP designation).

C8 chemistry is an alternative to the more widely-used C18 chemistry. C8 is of lower hydrophobicity than C18 materials. C8's lesser retention may help shorten total analysis time in the separation of compounds with large differences in hydrophobicity (where a C18 column might yield a long total analysis time), without requiring gradient elution.

Users of Triart are not limited by the usual constraints of pH, temperature, or pressure. Triart is recognized for its mechanical and chemical durability. It is ideal for application at elevated temperature, high pressure, and over a pH range of 1 to 12.

Triart is a dynamically growing line of hybrid materials designed for uHPLC, fast LC, analytical and prep HPLC. Triart C8 is offered in particle sizes from 1.9µm to 50µm; in a wide range of pre-packed columns as well as bulk media. Triart C8 1.9µm is offered packed in uHPLC columns and described separately, click here.

The product was developed incorporating expertise from YMC's microreactor research group to optimize the granulation of the particle itself.

YMC's high performance C8 chemistry delivers excellent peak shape under a wide range of separation conditions. Users can rely on Triart C8 reproducibility and retention consistency, not only across lots and batches, but across particle scales as well - from 1.9µm to 50 µm.

The chemistry of the C8 is enhanced by a multi-stage, multi-compound end-capping process that overcomes the weaknesses of single-step end-capping, and effectively caps essentially all the residual silanol sites that are accessible by analytes.

The combination of these core technologies - the layered hybrid particle, the precise microreactor granulation, and the multi-step endcapping - produce a material with outstanding chemical and physical durability, plus excellent chromatographic performance with acidic, basic, and neutral compounds.

This chemically stable and physically durable material offers chromatographers maximum flexibility in separation conditions over a wide pH range.

YMC-Triart C8 is ideal for use in method development and quality control.

 

• The chemical stability of the material combines with the inherent mechanical durability of the particles themselves, to contribute to long column lifetime.
• Triart is available in columns packed with 3 and 5 micron particles and operates over a wide pH range of 1 to 12, enabling the manipulation of analytical retention and preparative loading.
• Proprietary endcapping makes for excellent peak shapes for all types of compounds while rigorous manufacturing controls result in legendary YMC column to column and lot to lot reproducibility.
• Unlike other hybrid materials, YMC-Triart exhibits a more balanced hydrophobicity, making it more rententive than other hybrid materials, potentially simplifiying the separation of "difficult" peaks.
• YMC-Triart is available in packed columns of various dimensions. Bulk material is also available on request.

YMC-Triart columns are well-suited to a variety of applications in pharmaceutical and chemical laboratories. YMC-Triart's performance in wide-ranging conditions arguably makes it an ideal universal reversed-phase column choice.

Triart C8 has excellent durability in difficult conditions such as acidic or alkaline mobile phases, and at temperatures above ambient. Triart users - in method development or in routine use - are able to better optimize their separations using conditions normally thought to be "out of bounds"... and achieve repeatable success.

This advantage carries over into mainstream applications as well. The combined effects of pressure, temperature, and pH take their toll on conventional columns. Triart can be expected to outlast conventional columns by a factor of 10x or more.

High pH: Triethylamine, pH 11.5, 40°C

Column	:	5µm, 150 X 4.6 mm I.D.
Eluent	:	50mM triethylamine (pH 11.5)/methanol (90/10)
Flow rate	:	1.0 mL/min
Temperature	:	40°C
Sample	:	benzyl alcohol

Low pH, High Temperature: pH 1, 70°C

This column was stored in acetonitrile/water/RFA (10/90/1) at 70°C and tested every 20 hours using the conditions below.

Column	:	5µm, 50 X 2.0 mm I.D.
Eluent	:	acetonitrile/water (60/40)
Flow rate	:	0.2 mL/min
Temperature	:	37°C
Sample	:	butyl benzoate

This test demonstrates Triart C8's Ability to reproduce retention time under repeated injections conducted at pH 2 and 55°C. No change in retention time is observed through 400 injections, even under these difficult pH and temperature conditions.

Column	:	YMC-Triart C8 (5µm, 150 X 2.0 mm I.D.)
Eluent	:	Phosphate buffer (pH 2.0)/Acetonitrile (65/35)
Flow rate	:	0.28 mL/min
Temperature	:	55°C
Detection	:	UV at 240nm
Sample	:	butyl benzoate

Sample:

1. Hydrochlorothiazide
2. Amlodipine besilate
3. Valsartan
4. Atorvastatin calcium hydrate
5. Candesartan cilexetil

Column	:	YMC-Triart C8 (5µm, 50 X 2.0 mm I.D.)
Eluent	:	A) water/formic acid (100/0.1) B) acetonitrile/formic acid (100/0.1) 10-90%B (0-5 min), 90%B (5-7 min)
Flow rate	:	0.4 mL/min
Temperature	:	30°C
Detection	:	UV at 254nm
Injection	:	2µl (10-20µg/mL)

Triart C8 has different separation characteristics compared to Triart C18. Triart C8 would be useful for method optimization, especially in the cases where C18 phases are too retentive