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YMC-BioPro IEX

Ion exchange columns for separation of proteins, peptides, and nucleic acids.

YMC-BioPro IEX is a family of ion exchange (IEX) columns and bulk resins for analytical separations to benchtop and process/pilot-scale purifications of proteins, peptides, oligonucleotides, and other biomolecules.

BioPro IEX is based on a hydrophilic polymethacrylate bead which exhibits very low non-specific adsorption and may therefore reduce the need for organic solvents in the mobile phase.

These columns exhibit consistent retention, separation, and recovery, and are ideal for method validation due to proven lot-to-lot reproducibility.

Column Types

YMC-BioPro IEX columns are available with porous and non-porous particles for strong anion exchange (SAX) and strong cation exchange (SCX).

Columns packed with the porous materials (QA & SP) are advantageous for high-load analytical separations and laboratory-scale purification as they maintain strong peak shape even with increased load.

The non-porous formats (QF & SF) are highly efficient, and are therefore ideal for high-throughput analytical separations requiring high resolution.

SAX Columns

Product Name BioPro IEX QA BioPro IEX QF
Charged Group -CH2N+(CH3)3 -CH2N+(CH3)3
Porosity Porous Non-porous
pH Range 2-12 2-12
Particle Size 5µm 3µm, 5µm 6µm
Available Column Formats PEEK Hardware (Analytical) PEEK Hardware (Analytical) Stainless Steel Hardware (Prep)

SCX Columns

Product Name BioPro IEX SP BioPro IEX SF
Charged Group -CH2CH2CH2SO3 -CH2CH2CH2SO3
Porosity Porous Non-porous
pH Range 2-12 2-12
Particle Size 5µm 3µm, 5µm 6µm
Available Column Formats PEEK Hardware (Analytical) PEEK Hardware (Analytical) Stainless Steel Hardware (Prep)

Bulk Resin

YMC-BioPro IEX Resins

One of the advantages of YMC’s BioPro IEX resins is that they are fully scalable, exhibiting consistent chromatographic behavior across all particle sizes. Preparative grade resins with 10 – 75 μm particles are available in mL and L quantities.

YMC-BioPro IEX SmartSep Q/S (10, 20, or 30 μm), which show high resolution and recovery even at a high flow rate and high loading condition, are suitable for an intermediate purification step and a polishing step of mAbs, proteins, peptides, and oligonucleotides. YMC-BioPro IEX Q/S (75 μm) are designed for capture and intermediate purification of biopharmaceuticals.

YMC-BioPro IEX Resins

Screening Kits & MiniChrom® Columns

YMC-BioPro IEX Screening Kits

YMC-BioPro IEX Screening Kits contain five cartridges, each packed with 1mL or 5mL of 20μm, 30μm, or 75μm BioPro resin. These kits are ideal for media screening, development of purification methods, and loadability studies.

MiniChrom® BioPro Columns

MiniChrom® BioPro Columns are polypropylene LC columns packed with YMC-BioPro IEX stationary phase material. These columns are designed for operating conditions up to 30 °C and 30 bar (435 psi). They are useful for bench-scale resin cleaning and method development, sample cleaning, sample prep/cleanup, bind-and-recover sample purification, and flow-through purification.

Additional Resources

Brochures

Download the brochures below for more information about YMC-BioPro IEX products.

BioPro IEX Brochures



Applications
Care & Use Manuals

LC Columns & Accessories

ProductPhase / TypeSeparation Mode(s)Pore SizesParticle SizespH RangeAvailable FormatsLink to Order
YMC-BioPro IEXIEXIon Exchange (IEX)Non-Porous1000 Å3 µm5 µm10 µm20 µm75 µm6 µm30 µm2 - 12Analytical (1 - 8 mm ID)Preparative (10 mm ID & above)BiocompatibleScreening Kit

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