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Columns specifically designed for drug-to-antibody conjugates (ADCs) and antibodies.

YMC-BioPro HIC HT / BF are hydrophobic interaction chromatography (HIC) columns that utilize a butyl (C4) stationary phase bonded to non-porous hydrophilic polymer particles.

BioPro HIC HT offers higher resolution than conventional HIC columns and is the first choice for ADCs or mAbs.

BioPro HIC BF columns show a stronger retention and can therefore be used for the separation of low hydrophobic proteins or for the analysis of oxidized mAbs.

YMC-BioPro HIC columns are available in 4.6 mm ID.

HIC column


  BioPro HIC HT BioPro HIC BF
Base Particle hydrophilic polymer (polymethacrylate) hydrophilic polymer (polymethacrylate)
Particle Size (µm) 2.3 4
Pore non-porous non-porous
Functional Group butyl butyl
pH Range 2-12 2-12
Pressure Limit 40 MPa (5,800 psi) 20 MPa (2,900 psi)
Temperature Range 10 – 60° C 10 – 60° C

LC Columns & Accessories

ProductPhase / TypeSeparation Mode(s)Pore SizesParticle SizespH RangeAvailable FormatsLink to Order
YMC-BioPro HICC4Hydrophobic Interaction (HIC)Non-Porous4 µm2.3 µmAnalytical (1 - 8 mm ID)

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