YMC provides dynamic axial compression columns suited for laboratory-, pilot- and industrial-scale. These automated self packing columns allow for an easy, cost-effective refill of packing material that results in a column bed yielding superior durability and reproducibility. The DAD series operates using a downward-driving piston that maintains constant pressure on the bed during use and also simplifies packing and unpacking. The custom-designed DAR series employs a rotatable column and a water-pressure packing method.
In packing operation of axial compression columns, pressurizing by cylinder followed by loading slurry into columns can create homogenous packed bed. Packed bed pressurized by cylinder realizes high performance columns which are high densely and homogenously packed. Prevention of voids by constant pressurization also realizes long column life as a result of improvement of durability. (Axial Compression Technology)
The slurry container provides excellent distribution of packing material and allows for a “homogenous” slurry that can be automatically loaded into the DAD column by simply switching a valve. Throughout the operation from slurry preparation to filling slurry, safety and hygiene can be achieved.
The data on the right hand side shows repacking data obtained by using a 50 mm I.D. DAC column. After repacking the column 7 times, “plates/m” and “Tf” values are still as good as the initial state.
The chromatography on the right hand side shows a scale-up example from an analytical 4.6 mm I.D. column to a 50 mm I.D. DAC column. This indicates that the very similar separation pattern of analytical scale is also reproducible at the preparative scale.
Column: YMC-Pack C8 (S-5 μm, 200 Å)
150 X 4.6 mmI.D.
Flow rate: 0.8 mL/min
Column: YMC*GEL C8 (S-10 μm, 200 Å)
150 X 50 mmI.D.
Eluent: A) water/TFA (100/0.1)
B) acetonitrile/TFA (100/0.1)
25-60%B (0-20 min)
Flow rate: 100 mL/min
Detection: UV at 220 nm
DAD series : Driving direction of cylinder is downward. ( Catalog Download )
Packing & Testing the YMC 10cm Dynamic Axial Compression (DAU) Column
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