YMC Scale Up Platform Product Array

Evaluating 5' capping efficiency is a critical quality attribute in therapeutic mRNA manufacturing, directly impacting translation efficiency and transcript stability. However, separating structurally similar capped, partially capped, and uncapped species differing by just a single nucleotide remains a significant chromatographic challenge.

Ensuring the quality of single guide RNA (sgRNA) is a major hurdle in CRISPR-Cas genome editing. Because these molecules are large (~100mer) and prone to complex secondary structures, separating them from nearly identical impurities requires precision. Our latest application note

Accurate determination of 𝗽𝗲𝗿𝗳𝗹𝘂𝗼𝗿𝗼𝗼𝗰𝘁𝗮𝗻𝗲𝘀𝘂𝗹𝗳𝗼𝗻𝗶𝗰 𝗮𝗰𝗶𝗱 (𝗣𝗙𝗢𝗦) in food samples is challenging due to bile acid–related compounds (e.g., taurocholic acid isomers). These species can produce the same SRM transition (m/z 499→80) as PFOS, leading to potential false positives without adequate chromatographic separation.