Skip to main content
978-487-1100

Enrichment of Full AAV2 Using Multicolumn Countercurrent Solvent Gradient Purification (MCSGP)

Read our latest article on enhancing Adeno-associated virus (AAV) purity for gene therapy titled Enrichment of Full AAV2 Using Multicolumn Countercurrent Solvent Gradient Purification (MCSGP). AAVs are powerful delivery vehicles, but separating full (therapeutic) from empty capsids has been a significant hurdle.

This research introduces a twin-column multicolumn countercurrent solvent gradient purification (MCSGP) process that dramatically improves the ratio of full to empty capsids. The results are impressive:

  • Increased full capsid content: From 30% to 68% (ddPCR) and 27% to 61% (cryo-TEM)!
  • Overcame yield-purity trade-off: Enhanced full capsid recovery while boosting purity.
  • Improved productivity: Up to 23% increase compared to batch methods.
  • Reduced buffer consumption: Up to 27% decrease, offering economic and environmental benefits.

This innovative MCSGP process is a game-changer for meeting the growing demand for high-quality AAV products, pushing us closer to more effective and safer gene therapies.

Read the article here: https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/bit.29036

Posted on July 16, 2025.

Related Posts

August 6, 2026

Spanish – Vitaminas Complejo Vitaminico B

Las vitaminas son nutrientes esenciales para el mantenimiento de la salud y, por lo tanto, los productos que las contienen en su formulación requieren un riguroso control de calidad. Este proceso es fundamental para garantizar que la cantidad de vitaminas presente en un producto cumpla con las especificaciones establecidas, asegurando calidad y confiabilidad para los consumidores. Accede al enlace a continuación y descubre cómo complementar tu laboratorio para el análisis del complejo de vitamina B.
Read More

August 3, 2026

Portuguese – Vitaminas Complexo B

As vitaminas são nutrientes essenciais para a manutenção da saúde e, por isso, produtos que as contêm em sua formulação exigem um controle de qualidade rigoroso. Esse processo é fundamental para garantir que a quantidade de vitaminas presente no produto esteja de acordo com as especificações estabelecidas, assegurando qualidade e confiabilidade ao consumidor. Clique no link abaixo e confira como suplementar o seu laboratório para a análise de vitaminas do complexo B.
Read More

July 30, 2026

Two-Step RP Purification of Tirzepatide

Scaling Up Tirzepatide Purification: From 20% Crude to >99% Purity With the surge in demand for GLP-1 receptor agonists, efficient and scalable purification strategies for complex lipopeptides like Tirzepatide have never been more critical.
Read More

Designed and developed by