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Hydrosphere C18

Provides superior separation of hydrophilic compounds.

Hydrosphere C18 is based on highly inert, ultra-pure, pH neutral silica, and is designed to maintain a hydrophilic surface for superior separation of hydrophilic compounds. Minimal metal content and very low concentrations of residual silanols make this an ideal phase for the analysis of basic pharmaceuticals and chelating compounds, even under LC-MS conditions such as separation in non-phosphate buffered systems. In addition, the ability to separate polar compounds under highly aqueous mobile phase conditions without loss in retention, plate counts, and peak performance greatly enhances the versatility of Hydrosphere C18.


  • Made with highly inert, ultrapure, pH neutral silica
  • Hydrophilic C18 surface for enhanced polar selectivity
  • Extremely rigorous physical and chemical specifications
  • Suitable for conventional reversed phase and highly aqueous applications
  • Suitable for pharmaceutical method development


Typical applications include: strong polar compounds, water-soluble vitamins


Stationary Phase C18 (USP L1)
Bonding Monomeric
Particle Size 2, 3, 5 µm
Pore Size 120 Å
Carbon Content 12%
pH Range 2 – 8
Temperature Upper Limit 50 °C
Temperature Recommended Range 20 – 40 °C

Available Formats

  • Prepacked columns & guards
    • Capillary (< 1 mm ID)
    • Analytical (1.0 – 8.0 mm ID)
    • Preparative (10 mm ID & above)

LC Columns & Accessories

ProductPhase / TypeSeparation Mode(s)Pore SizesParticle SizespH RangeAvailable FormatsLink to Order
Hydrosphere C18C18Reversed Phase120 Å3 µm5 µm2 µm2 - 8Analytical (1 - 8 mm ID)Capillary (< 1 mm ID)Preparative (10 mm ID & above)

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