Insulin is a peptide hormone. Due to the typical scale of insulin production and market pressures to increase availability and reduce the cost of insulin, there is justification to invest in finding improved conditions and materials for insulin purification.
In partnership with a leading insulin manufacturer, YMC projected the cost contributions by the three principal cost drivers associated with preparative LC processes: labor/overhead comprises roughly 56% of the cost; mobile phase consumption accounts for 31%; and stationary phase expense is the smallest factor, accounting for the remaining 13%.
Ironically, it is the stationary phase that can exert the most significant impact on the other two cost drivers (processing time and mobile phase consumption)—the lowest contributor to cost has the greatest impact on overall cost-effectiveness.
In the case study, several stationary phases were tested (all of them C8 reversed-phase). Based on the tests, the study included a careful cost estimation for the isolation of 100kg of purified insulin.
Key factors in the cost projections included:
YMC-Triart Prep Bio200 C8 outperformed all other phases in the test, not just in overall projection but in each individual metric.
Furthering our leadership in insulin purification, YMC’s CaptureSMB technology will increase the yield up to 80% without compromising target purity and will reduce affinity resin costs by up to 60% without changing its use in either the Cascade or the Cohn fractionation process.
YMC technology employing the MCSGP process principle
Learn more about this and other insulin purification approaches by downloading our Case Study: YMC Contichrom in Blood Plasma Fractionation.
YMC-Triart Prep Bio200 CB
Insulin Purification Case Study
YMC – What We Do
Modified Cohn Process using CaptureSMB and MCSGP to optimize yield and purity for high value plasma proteins
Contichrom in Blood Plasma Fractionation
Simulation, optimization and implementation of a twin-column MCSGP process for the purification of Liraglutide
UC-based dynamic process control of twin-column LPLC system
mAb isoform profile tuning obtaining a biobetter productbiobetter productusing the Contichrom CUBE FPLC Systemwith high productivity and load
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