What is Hydrophobic Interaction Chromatography? The All-New BioPro HIC BF Column

If you are interested in learning more about our high-performance liquid chromatography products online, we encourage you to browse our website today. Continue below to learn about our new hydrophobic interaction chromatography (HIC) columns and how they can help improve your protein separations:

What is HIC?

The technique known as hydrophobic interaction chromatography (HIC) is a mode of chromatography that separates proteins by differences in surface hydrophobicity. This method utilizes reversible interactions that occur between protein molecules and hydrophobic stationary phase ligands attached to the resin surface.

Certain non-denaturing salts (such as ammonium sulfate or sodium chloride) are used to improve the hydrophobic interactions between proteins and the stationary phase. In most cases, these phases use silica, highly cross-linked agarose, or polystyrene-divinylbenzene as the base particle. Hydrophobic functional groups such as phenyl, ether, octyl (C8), butyl (C4), or amide (among others) are bonded to these resins and interact with the hydrophobic ends of proteins. A reverse gradient is typically run from high-to-low salt concentration in order to effect separation based on relative hydrophobicity.

The All-New BioPro HIC BF

YMC BioPro HIC BF is a newly-developed hydrophilic polymer stationary phase bonded with C4 (butyl).  Its 4µm, non-porous particle provides high mechanical stability along with high column efficiency, allowing for fast analysis at higher flow rates. BioPro HIC BF is particularly effective when it comes to separating proteins and antibodies, specifically when running drug-to-antibody ratio (DAR) analyses on antibody-drug conjugates (ADCs).

YMC manufactures BioPro HIC BF columns under strict quality control conditions, and every column must pass stringent release testing prior to shipment. We strive to make sure that we produce nothing but the highest quality products for our clients, as we have for more than 35 years.

Contact Us   

To find out more about this brand new HIC column or our high-performance liquid chromatography products online, we encourage you to continue browsing our website or call 888-341-8380 to speak with a representative.