Normal Phase Chromatography Solutions
High-Performance Columns and Resins for Polar Compound Separations
Normal phase chromatography (NP) is a powerful technique used to separate compounds based on polarity, ideal for analytes that are more soluble in organic solvents or require separation by hydrophilic interaction. In this mode, the stationary phase is polar, and the mobile phase is non-polar. Polar compounds interact more strongly with the stationary phase and are retained longer, while less polar or non-polar compounds move through the column more quickly.
YMC offers a versatile portfolio of normal phase columns and resins built on high-purity silica, and functionalized with dihydroxypropyl, aminopropyl, polyamine, and other chemistries. Whether you’re working with lipids, sugars, steroids, isomers, or chiral compounds, YMC products deliver repeatable, reliable results from lab to production scale.
The table below gives an overview of YMC’s products for normal phase chromatography. For more specific information, including applications and purchase options, click the product name.
| Product Name | Phase | Particle Size (µm) | Pore Size (Å) | USP | pH Range | Typical Applications | Characteristics |
|---|---|---|---|---|---|---|---|
| YMC-Pack Diol-NP & Diol-NP-HG | dihydroxypropyl | 5, 10, 15, 20, 50 | 60, 120, 200, 300 | L20 | 2-7.5 | pharmaceutical products, metabolites, phospholipid classes | normal phase separation using non-polar solvents, useful for hydrophilic interaction chromatography (HILIC) |
| YMC-Pack NH2 & NH2-HG | aminopropyl | 5, 10, 15, 20, 50 | 120, 200, 300 | L8 | 2-7.5 | saccharides, nucleotides, water-soluble vitamins | primary amino derivative, high coverage monomeric bonding chemistry, suitable for HILIC |
| YMC-Pack PA-G | polyamine | 5 | 120 | – | 4-7.5 | acidic oligosaccharides | higher durability than conventional silica-based amino columns |
| YMC-Pack Polyamine II | polyamine | 5 | 120 | L111 | 2-7.5 | sugars, vitamins, fat-soluble compounds | higher durability than conventional silica-based amino columns |
| YMC-Pack SIL & SIL-HG | silica | 3, 5, 10, 15, 20, 50 | 60, 80, 120, 200, 300 | L3 | 2-7.5 | steroids, fat-soluble vitamins, small organic molecules, tocopherols | high grade silica |
| YMC-Pack PVA-Sil | polyvinyl alcohol | 5 | 120 | L24 | 2-9.5 | phospholipid classes | high stability & reproducibility |
| YMC-Triart SIL | bare silica | 3, 5 | 120 | L3 | 2-8 | nucleic acids, amino acids, peptides, proteins, drugs, metabolites | bare organic/inorganic hybrid silica column with superior chemical stability |
| YMC-Triart Prep SIL | bare silica | 10, 15, 20 | 120 | L3 | 2-8 | nucleic acids, amino acids, peptides, proteins, drugs, metabolites | bare organic/inorganic hybrid silica column with superior chemical stability |
When to Use Normal Phase Chromatography
Normal phase separation is ideal when:
- Reversed-phase chromatography is ineffective as with very polar compounds, which tend to be poorly retained.
- Compounds are more soluble in organic solvents
- Separating structurally similar compounds with polar functionalities
Why Choose YMC?
Reproducibility You Can Rely On
Our columns and resins are batch-tested and engineered to deliver consistent performance, even across different production lots.
From Method Development to Production Scale
YMC supports your workflow across the entire lifecycle, from lab-scale method development to custom skid systems and preparative-scale purification.
Custom Solutions, Not Commodities
Unlike off-the-shelf options, YMC offers tailored separation products designed around your application, not the other way around.
Hybrid Silica Innovation
Our hybrid silica columns combine the best of organic and inorganic materials, giving you greater chemical stability and longer product life.
Normal Phase Chromatography FAQs
Q: Can YMC normal phase columns be used for HILIC separations?
Yes. Several of our phases (e.g., dihydroxypropyl, aminopropyl) are suitable for hydrophilic interaction chromatography and can handle both normal phase and HILIC workflows.
Q: What is the pH range of YMC’s normal phase columns?
Most YMC normal phase products operate effectively between pH 2 – 7.5, with some reaching up to pH 9.5. Always refer to individual product specs.
Q: What solvents are compatible with YMC’s normal phase materials?
Our columns are compatible with non-polar organic solvents commonly used in NPC, including hexane, chloroform, and isopropanol. Cleaning protocols may vary. Contact us for recommendations.
Need Help Choosing the Right Column?
Our team of experts can assist with selecting the right stationary phase and format for your specific separation needs. YMC also offers custom packing and scale-up solutions for biopharma and analytical workflows.
Contact us today or browse our selection of columns.
