Continuous Purification: CaptureSMB

Continuous purification: MCSGP and CaptureSMB—
industry disruptive technologies

Continuous production of peptides, oligos, and large biomolecules is a growing trend finding favor with regulatory bodies for the ability of continuous manufacturing to make drugs of a higher and more consistent quality at a lower cost and thus more readily accessible. Continuous purification with chromatography is widely defined as a process that moves beyond the present batch time to runs that extend into days, weeks, or months. YMC is a pioneer in the technology that semi or continuously uses chromatographic processes to purify drug products.

YMC has supplied twin-column LPLC and HPLC “Contichrom” systems for major pharmaceutical and bio-pharmaceutical GMP suites in Europe, the USA, and Asia. These systems range from a few liters per minute to over 40 LPM purifying tonnes of advanced therapeutics. YMC is recognized as the industry leader in continuous and semi-continuous chromatography-based purification technology.

YMC has patented the industry-leading continuous purification approaches of CaptureSMB for biologics and MCSGP for peptides and oligonucleotides. Read more about these disruptive technologies below.

Continuous CaptureSMB processes—biologics

During the production of monoclonal antibodies, the Protein A capture step is the first important purification step. A fast capture step is critical to retaining product integrity and processing of large feed volumes in reasonable times. Novel periodic counter-current (PCC) processes are superior to batch processes, offering a significant economic benefit. 

YMC/ChromaCon’s optimized two-column periodic countercurrent chromatography process (CaptureSMB) is the least complex and most effective process, allowing two to threefold faster processing. It saves up to 60% of expensive Protein A resin and buffer. Lab (Contichrom CUBE instrument) and GMP scale production systems (Contichrom TWIN LPLC) employing CaptureSMB are commercially available.

CaptureSMB resources

The CaptureSMB Process Principle

Maximizing the efficiency of affinity capture steps

View: Presentation

CaptureSMB for the continuous purification of mAbs with high productivity and load

CaptureSMB is a twin-column periodic countercurrent (PCC) process used for the continuous purification of monoclonal antibodies (mAbs) and antibody fragments using affinity resins such as Protein A and Protein L.

Read: White Paper

Capture SMB; Twin-column chromatography—how it works, economics, and applications

This 25-minute presentation (plus 15-minute Q&A) walks through how a twin-column chromatography system works, both at the bench and production scale.

Watch: Webinar

More Resources Related to This Topic

Application Information

Technology and Economics for the Purification of Oligonucleotides (and Peptides) by Twin-Column Continuous Chromatography

Process Intensification for the Purification of Peptidomimetics: The Case of Icatibant through Multi-column Countercurrent Solvent Gradient Purification (MCSGP)

Comparison of Multicolumn Capture Processes

Process Control and Optimization by AutomAb

Dynamic Process Control (MControl®) for Countercurrent Polish Processes (MCSGP)

Application Search

Use our extensive Applications Search page to mine a dynamically growing collection of over 350 YMC-produced application notes.

The data can be searched by any combination of several variables, including sample classification, compound name (including partial names), and column parameters.

Contact YMC America

For more information on products and services from YMC, YMC ChromaCon, or just to say hello, get in touch with us by clicking on the button below.