Continuous Purification: CaptureSMB

Continuous CaptureSMB processes—biologics


During the production of monoclonal antibodies, the Protein A capture step is the first important purification step. A fast capture step is critical to retaining product integrity and processing of large feed volumes in reasonable times. Novel periodic counter-current (PCC) processes are superior to batch processes, offering a significant economic benefit. 

YMC/ChromaCon’s optimized two-column periodic countercurrent chromatography process (CaptureSMB) is the least complex and most effective process, allowing two to threefold faster processing. It saves up to 60% of expensive Protein A resin and buffer. Lab (Contichrom CUBE instrument) and GMP scale production systems (Contichrom TWIN LPLC) employing CaptureSMB are commercially available.

CaptureSMB resources

The CaptureSMB Process Principle

Maximizing the efficiency of affinity capture steps

View: Presentation

CaptureSMB for the continuous purification of mAbs with high productivity and load

CaptureSMB is a twin-column periodic countercurrent (PCC) process used for the continuous purification of monoclonal antibodies (mAbs) and antibody fragments using affinity resins such as Protein A and Protein L.

Read: White Paper

Capture SMB; Twin-column chromatography—how it works, economics, and applications

This 25-minute presentation (plus 15-minute Q&A) walks through how a twin-column chromatography system works, both at the bench and production scale.

Watch: Webinar

More Resources Related to This Topic

Application Information

Technology and Economics for the Purification of Oligonucleotides (and Peptides) by Twin-Column Continuous Chromatography

Process Intensification for the Purification of Peptidomimetics: The Case of Icatibant through Multi-column Countercurrent Solvent Gradient Purification (MCSGP)

Comparison of Multicolumn Capture Processes

Process Control and Optimization by AutomAb

Dynamic Process Control (MControl®) for Countercurrent Polish Processes (MCSGP)

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