Reversed Phase Chromatography Columns
Products for Reversed Phase Separation
Versatile separation solutions for small molecules, peptides, proteins, and more.
Reversed-phase liquid chromatography (RP-LC) is the most widely used mode of HPLC/UHPLC due to its ability to separate a broad range of compounds based on hydrophobic interactions. YMC’s portfolio of reversed-phase columns combines high chemical stability, reproducibility across lots, and scalability from method development to production.
Whether you’re working with APIs, peptides, proteins, or complex mixtures, YMC offers C18, C8, C4, phenyl, cyano, and specialty phases engineered to deliver consistent, high-resolution separations.
Selecting the Right Reversed Phase Column
YMC’s reversed-phase portfolio is built on high-purity silica and hybrid silica materials, available in a wide range of pore sizes, particle sizes, and chemistries to match your analyte and method needs.
Hybrid Silica Particle
| Product Name | Phase | USP | Pore Size (Å) | Particle Size (µm) | Carbon Content (%) | pH Range | Bonding | Endcapping | Typical Applications |
|---|---|---|---|---|---|---|---|---|---|
| C18 | L1 | 120 | 1.9, 3, 5 | 20 | 1 – 12 | trifunctional | multi-stage | acidic, neutral, basic compounds, medium polar compounds, peptides, oligonucleotides, small molecules, amino acids, APIs, sugars | |
| C18 | L1 | 120 | 7, 10, 15, 20 | 20 | 2 – 10 | trifunctional | multi-stage | acidic, neutral, basic compounds, peptides, oligonucleotides, small molecules, amino acids, APIs, sugars | |
| C18 | L1 | 80 | 1.9, 3, 5 | 25 | 1 – 12 | trifunctional | multi-stage | stereoisomers and hydrophobic analytes, fatty acids | |
| C8 | L7 | 120 | 1.9, 3, 5 | 17 | 1 – 12 | trifunctional | multi-stage | acidic, neutral, basic compounds | |
| YMC-Triart Prep C8-S | C8 | L7 | 120 | 10, 15, 20 | 17 | 2 – 10 | trifunctional | multi-stage | acidic, neutral, basic compounds, peptides, oligonucleotides, small molecules, amino acids, APIs |
| YMC-Triart Prep Bio200 C8 | C8 | L7 | 200 | 10 | 14 | 2 – 10 | trifunctional | multi-stage | peptides (especially insulin, insulin-like peptides), oligonucleotides, proteins |
| C4 | L26 | 300 | 1.9, 3, 5 | – | 1 – 10 | trifunctional | multi-stage | peptides, proteins, antibodies | |
| C18 | L1 | 300 | 1.9, 3, 5 | – | 1 – 12 | trifunctional | multi-stage | acidic, neutral, basic compounds | |
| pentafluorophenyl | L43 | 120 | 1.9, 3, 5 | 15 | 1 – 8 | trifunctional | none | aromatic stereoisomers, halogenated and polar compounds | |
| phenylbutyl | L11 | 120 | 1.9, 3, 5 | 17 | 1 – 10 | trifunctional | multi-stage | aromatic compounds, pharmaceuticals, sweeteners | |
| phenylbutyl | L11 | 120 | 10 | 17 | 2 – 10 | trifunctional | multi-stage | acidic, neutral, basic compounds, peptides, small proteins, phenols, aromatics | |
| YMC-Triart Prep C4-S | C4 | L26 | 120 | 10 | 14 | 2 – 10 | trifunctional | multi-stage | non-polar peptides |
100% aqueous stable (no phase de-wetting for reversed phase)
Larger particle sizes available for direct scale-up
C18 Products
| Product Name | Phase | USP | Pore Size (Å) | Particle Size (µm) | Carbon Content (%) | pH Range | Bonding | Endcapping | Typical Applications |
|---|---|---|---|---|---|---|---|---|---|
| C18 | L1 | 120 | 1.9, 3, 5 | 20 | 1 – 12 | trifunctional | multi-stage | acidic, neutral, basic compounds, medium polar compounds, peptides, oligonucleotides, small molecules, amino acids, APIs, sugars | |
| YMC-Triart C18 ExRS | C18 | L1 | 80 | 1.9, 3, 5 | 25 | 1 – 12 | trifunctional | multi-stage | stereoisomers and hydrophobic analytes, fatty acids |
| C18 | L1 | 300 | 1.9, 3, 5 | – | 1 – 12 | trifunctional | multi-stage | acidic, neutral, basic compounds | |
| C18 | L1 | 120 | 7, 10, 15, 20 | 20 | 2 – 10 | trifunctional | multi-stage | acidic, neutral, basic compounds, peptides, oligonucleotides, small molecules, amino acids, APIs, sugars | |
| Hydrosphere C18 | C18 | L1 | 120 | 2, 3, 5 | 12 | 2 – 8 | Monomeric | Yes | Strong polar compounds, water-soluble vitamins |
| J’Sphere ODS-H80 | C18 | L1 | 80 | 4 | 22 | 1 – 9 | Polymeric | Yes | positional isomers, complexing agents |
| J’Sphere ODS-L80 | C18 | L1 | 80 | 4 | 9 | 2 – 7.5 | Polymeric | Yes | positional isomers, complexing agents |
| J’Sphere ODS-M80 | C18 | L1 | 80 | 4 | 14 | 2 – 7.5 | Monomeric | Yes | positional isomers, complexing agents |
| Meteoric Core C18 | C18 | L1 | 80 | 2.7 | 7 | 1.5 – 10 | trifunctional | Yes | fast analysis of basic and coordinating compounds |
| Meteoric Core C18 BIO | C18 | L1 | 160 | 2.7 | 5 | 1.5 – 10 | trifunctional | Yes | fast analyses of peptides and small proteins, ideal for peptide mapping |
| YMC-Pack ODS-A | C18 | L1 | 120, 200, 300 | 3, 5 | 17, 12, 7 | 2 – 7.5 | Monomeric | Yes | validated API methods |
| YMC-Pack ODS-A-HG | C18 | L1 | 120, 200, 300 | 10, 15, 20, 50 | 17, 12, 7 | 2 – 7.5 | – | – | validated API methods |
| YMC-Pack ODS-AL | C18 | L1 | 120 | 5 | 17 | 2 – 7.5 | Monomeric | No | tocopherols, fat-soluble vitamins, disinfectants |
| YMC-Pack ODS-AM | C18 | L1 | 120 | 3, 5 | 17 | 2 – 7.5 | Monomeric | Yes | purines, phenols, alkaloids |
| YMC-Pack ODS-AQ | C18 | L1 | 120, 200 | 3, 5 | 14, 10 | 2 – 7.5 | Monomeric | Yes | strong polar compounds |
| YMC-Pack ODS-AQ-HG | C18 | L1 | 120, 200 | 10, 15, 20, 50 | 14, 10 | 2 – 7.5 | – | – | strong polar compounds |
| YMC-Pack Pro C18 | C18 | L1 | 120 | 2, 3, 5, 10 | 16 | 2 – 8 | Monomeric | Yes | Antioxidants, metabolites, APIs |
| YMC-Pack Pro C18 RS | C18 | L1 | 80 | 3, 5 | 22 | 1 – 10 | Polymeric | Yes | Stereoisomers and hydrophobic analytes |
100% aqueous stable (no phase de-wetting for reversed phase)
Larger particle sizes available for direct scale-up
C8 Products
| Product Name | Phase | USP | Pore Size (Å) | Particle Size (µm) | Carbon Content (%) | pH Range | Bonding | Endcapping | Typical Applications |
|---|---|---|---|---|---|---|---|---|---|
| C8 | L7 | 120 | 1.9, 3, 5 | 17 | 1 – 12 | trifunctional | multi-stage | acidic, neutral, basic compounds | |
| YMC-Triart Prep C8-S | C8 | L7 | 120 | 10, 15, 20 | 17 | 2 – 10 | trifunctional | multi-stage | acidic, neutral, basic compounds, peptides, oligonucleotides, small molecules, amino acids, APIs |
| YMC-Triart Prep Bio200 C8 | C8 | L7 | 200 | 10 | 14 | 2 – 10 | trifunctional | multi-stage | peptides (especially insulin, insulin-like peptides), oligonucleotides, proteins |
| YMCbasic | C8 | L7 | 200 | 3, 5, 10, 15, 20 | 7 | 2 – 7.5 | Monomeric | Yes | basic molecules, anilines, alkaloids, antidepressants |
| Meteoric Core C8 | C8 | L7 | 80 | 2.7 | 5 | 1.5 – 9 | trifunctional | Yes | n-terminal sequencing |
| YMC-Pack C8 | C8 | L7 | 120, 200, 300 | 3, 5 | 10, 7, 4 | 2 – 7.5 | Monomeric | Yes | proteins and peptides, estrogens |
| YMC-Pack C8-HG | C8 | L7 | 120, 200, 300 | 10, 15, 20, 50 | 10, 7, 4 | 2 – 7.5 | Monomeric | Yes | proteins and peptides, estrogens |
| YMC-Pack Pro C8 | C8 | L7 | 120 | 3, 5 | 10 | 2 – 7.5 | Monomeric | Yes | Acidic, neutral, basic and chelating compounds, drugs/ metabolites |
Larger particle sizes available for direct scale-up
C4 Products
| Product Name | Phase | USP | Pore Size (Å) | Particle Size (µm) | Carbon Content (%) | pH Range | Bonding | Endcapping | Typical Applications |
|---|---|---|---|---|---|---|---|---|---|
| C4 | L26 | 300 | 1.9, 3, 5 | – | 1 – 10 | trifunctional | multi-stage | peptides, proteins, antibodies | |
| YMC-Triart Prep C4-S | C4 | L26 | 120 | 10 | 14 | 2 – 10 | trifunctional | multi-stage | non-polar peptides |
| YMC-Pack C4 | C4 | L26 | 120, 200, 300 | 3, 5 | 7, 5, 3 | 2 – 7.5 | Monomeric | Yes | biological separations, polar compounds |
| YMC-Pack C4-HG | C4 | L26 | 120, 200, 300 | 10, 15, 20, 50 | 7, 5, 3 | 2 – 7.5 | Monomeric | Yes | biological separations, polar compounds |
| YMC-Pack Pro C4 | C4 | L26 | 120 | 3, 5 | 7 | 2 – 7.5 | Monomeric | Yes | Polar acidic, neutral, basic and chelating compounds |
100% aqueous stable (no phase de-wetting for reversed phase)
Coreshell
| Product Name | Phase | USP | Pore Size (Å) | Particle Size (µm) | Carbon Content (%) | pH Range | Bonding | Endcapping | Typical Applications |
|---|---|---|---|---|---|---|---|---|---|
| Meteoric Core C18 | C18 | L1 | 80 | 2.7 | 7 | 1.5 – 10 | trifunctional | Yes | fast analysis of basic and coordinating compounds |
| Meteoric Core C18 BIO | C18 | L1 | 160 | 2.7 | 5 | 1.5 – 10 | trifunctional | Yes | fast analyses of peptides and small proteins, ideal for peptide mapping and |
| Meteoric Core C8 | C8 | L7 | 80 | 2.7 | 5 | 1.5 – 9 | trifunctional | Yes | n-terminal sequencing |
Phenyl Phases
| Product Name | Phase | USP | Pore Size (Å) | Particle Size (µm) | Carbon Content (%) | pH Range | Bonding | Endcapping | Typical Applications |
|---|---|---|---|---|---|---|---|---|---|
| phenylbutyl | L11 | 120 | 1.9, 3, 5 | 17 | 1 – 10 | trifunctional | multi-stage | aromatic compounds, pharmaceuticals, sweeteners | |
| pentafluorophenyl | L43 | 120 | 1.9, 3, 5 | 15 | 1 – 8 | trifunctional | none | aromatic stereoisomers, halogenated and polar compounds | |
| phenylbutyl | L11 | 120 | 10 | 17 | 2 – 10 | trifunctional | multi-stage | acidic, neutral, basic compounds, peptides, small proteins, phenols, aromatics | |
| YMC-Pack Ph | Phenyl | L11 | 120 | 3, 5 | 9 | 2 – 7.5 | Monomeric | Yes | aromatic compounds, phenols, fullerenes, sweeteners |
| YMC-Pack Ph-HG | Phenyl | L11 | 120, 200, 300 | 10, 15, 20, 50 | 9 | 2 – 7.5 | Monomeric | Yes | aromatic compounds, phenols, fullerenes, sweeteners |
100% aqueous stable (no phase de-wetting for reversed phase)
Larger particle sizes available for direct scale-up
Specialty Phases
| Product Name | Phase | USP | Pore Size (Å) | Particle Size (µm) | Carbon Content (%) | pH Range | Bonding | Endcapping | Typical Applications |
|---|---|---|---|---|---|---|---|---|---|
| YMC Carotenoid | C30 | L62 | proprietary | 3, 5 | proprietary | 2 – 7.5 | Polymeric | Yes | isomeric carotenes, retinols, steroids, fat-soluble vitamins |
| YMC-Pack CN | Cyano | L10 | 120, 300 | 3, 5 | 7, 3 | 2 – 7.5 | Monomeric | Yes | steroids, catechols |
| YMC-Pack CN-HG | Cyano | L10 | 120, 200, 300 | 10, 15, 20, 50 | 7, 3 | 2 – 7.5 | Monomeric | Yes | steroids, catechols |
| YMC-Pack TMS | C1 | L13 | 120 | 3, 5 | 4 | 2 – 7.5 | Monomeric | Yes | water-soluble vitamins |
| YMC-Pack TMS-HG | C1 | L13 | 120, 200, 300 | 10, 15, 20, 50 | 4 | 2 – 7.5 | Monomeric | Yes | water-soluble vitamins |
| YMC PAH | proprietary | L118 | proprietary | 3, 5 | proprietary | 2 – 6.5 | – | none | PAHs, PCBs |
How Reversed-Phase Chromatography Works
In reversed-phase HPLC, the stationary phase is non-polar (hydrophobic), typically made of C18, C8, C4, PFP, and Phenyl bonded to silica. The mobile phase is polar, usually a mixture of water with methanol or acetonitrile. Hydrophobic compounds interact more strongly with the stationary phase and are retained longer, while more polar compounds elute sooner.
While hydrophobic partitioning is the primary mechanism of retention in reversed-phase chromatography, several secondary interactions can significantly impact selectivity and resolution, such as:
- Hydrogen Bonding – Between analyte functional groups (e.g., hydroxyls, amines) and residual silanols or bonded phases
- Dipole–Dipole Interactions – Important for separating polar neutral compounds
- π–π Interactions – Especially relevant for aromatic and ring-containing compounds interacting with phenyl or fluorinated phases
These secondary interactions help fine-tune separations, particularly in complex mixtures, isomeric compounds, and challenging analyte classes.
Applications of Reversed-Phase Chromatography
YMC’s reversed-phase columns are used across a variety of industries and compound types:
- Pharmaceuticals and APIs – Analysis of drug substances, metabolites, and formulations
- Proteins & Peptides – RP-LC is widely used for peptide mapping, biomolecule purification, and QC
- Environmental & Food Testing – Monitoring for contaminants, pollutants, and residues
- PFAS Analysis – Detection of per- and polyfluoroalkyl substances in environmental and food matrices
- Metabolomics, Bioanalysis & Biomarker Discovery – Profiling of complex biological samples, small molecules, and biomarkers
Why Labs Choose YMC
Reproducibility You Can Trust
YMC’s proprietary manufacturing processes ensure consistent performance across lots, critical for regulated workflows and validated methods.
Full Lifecycle Support
Our columns are available in analytical, semi-prep, and preparative formats, making it easy to scale from R&D to production.
Custom Separation Solutions
YMC doesn’t just sell stock columns; we design and pack columns tailored to your separation challenges and system requirements.
Stable Under Harsh Conditions
Hybrid silica and polymeric bonding options withstand aggressive pH conditions, temperature variations, and complex sample matrices.
Reversed Phase Chromatography FAQs
Q: What’s the difference between C18 and C8 columns?
C18 and C8 columns both use bonded alkyl chains as the stationary phase, but differ in chain length. C18 columns have longer alkyl chains, which provide greater hydrophobicity and stronger retention of non-polar compounds. C8 columns, with shorter alkyl chains, offer reduced retention, making them better suited for compounds with moderate hydrophobicity or when shorter run times are desired.
Q: Can YMC columns be used at high pH?
Yes, many of our hybrid silica and polymer-bonded columns are stable at elevated pH (up to 12), depending on the phase. Please consult product specs for pH range.
Q: Which column is best for separating proteins and peptides?
C4 and wide-pore C18/C8 phases are commonly used for peptide and protein separation, including oligonucleotides, insulin, and monoclonal antibodies.
Q: Are YMC phases available in core-shell formats?
Yes. YMC offers core-shell options for faster separations with high efficiency and reduced solvent consumption.
Start Your Separation With Confidence
Need help selecting the right reversed-phase column for your application? YMC’s chromatography experts are here to guide you. From method development to scale-up, we deliver solutions, not just columns.
Contact us today or browse our selection of columns.
