Phenyl columns provide unique selectivity when compared to aliphatic straight chain reversed-phase materials such as C18, C8, and C4. The π electrons of the phenyl group can interact with the aromatic ring(s) of an analyte to increase retention relative to non-aromatic species. Phenyl also has application as an alternative to C18 and C4 for the analysis of peptides and proteins on both small-pore (120Å) and wide-pore (300Å) silica supports. Retention is decreased on wide-pore phenyl phases relative to 120Å material. Generally, as pore size increases, surface area decreases, resulting in shorter retention times.
Guard columns are recommended for challenging matrices or for use as trapping columns. YMC capillary guard columns are connected directly to the analytical column using the supplied column coupler.
A column coupler is supplied with every pack of YMC capillary guard cartridges to guarantee an optimum connection with low dead volume. A polymer-based (PCTFE) coupler is provided for 1/16” columns, while a stainless steel coupler is provided for 1/32” columns. Additional couplers can be purchased separately if required.
Columns, Guards and Accessories are only available for purchase online in North America or through our distributors.
To purchase consumables outside North America, please find your local distributor.
For YMC systems’ sales outside of North America and Europe, find your regional office contact information here.