Reversed-Phase Chromatography

Unlike normal phase chromatography, in which the stationary phase is hydrophilic, reverse phase chromatography employs a stationary phase that is hydrophobic in nature. As a result, molecules with hydrophobic properties contained within the mobile phase or feedstock will have a high affinity for the stationary phase and will more readily adhere to the column packing.

Hydrophilic molecules having less of an affinity for the column packing will pass through or be eluted first. Hydrophobic molecules can be eluted from the column by decreasing the polarity of the mobile phase using an organic (non-polar) solvent. The non-polar nature of the solvent reduces hydrophobic interactions and allows release from the stationary phase so it can be collected. The more hydrophobic the molecule, the more strongly it will bind to the stationary phase, and the higher the concentration of organic solvent that will be required to elute the molecule.

YMC has been a manufacturer of reverse phase technology for nearly 40 years having pioneered uses in industrial chromatography. Discover the many YMC reverse phase products that offer the user superior separations under a wide range of conditions.


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