Ion exchange (IEX) chromatography is based on the principle of the separation of molecules on the basis of their charge. IEX technique leverages the interaction between charged molecules in the feedstock (or mobile phase) and oppositely charged sites on the stationary phase (or chromatography resin). IEX is a technique that is frequently used in (charged) biomolecule purification.
Cation exchange and anion exchange are two types of ion exchange separation. In anion exchange, the stationary phase is positively charged, while in cation exchange, it is negatively charged.
The molecules separated on the basis of their charge are eluted using a buffer solution with varying ionic strength. By flowing buffer over the resin, a selective separation of molecules according to their different charges takes place.
For most therapeutic proteins, YMC offers an IEX pre-packed column or bulk resin that provides the user with exceptional performance vs. conventional IEX media. The combination of YMC LPLC systems and IEX bulk resin provides a complete, high-performance Ion Exchange solution for modern biopharmaceuticals.
Visit the individual product pages for more information:
Making size exclusion chromatography (SEC) scalable using Contichrom
Optimization of oligonucleotide separations on ion-exchange chromatography
YMC-BioPro Preparative Screening Kits
A newly developed hydrophilic polymer-based ion exchange chromatography media and purification of immunoglobulin Y from egg yolk
A newly developed hydrophilic polymer-based ion exchange resin for analysis and purification of various biological molecules
Downstream process development of monoclonal antibodies in high-yield and high-purity by affinity and ion-exchange chromatography 2019
Developments of Hydrophilic Polymer-based Ion Exchange Media for Analysis and Purification of Various Biological Molecules
An Evaluation of YMC’s Strong Cation-Exchange Stationary Phase for the Analysis of Charged Isoforms of a Commercially Available Antibody Drug
YMC Insight Center CEO Perspective on Lab Services
YMC Insight Center – A Virtual Tour
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