LPLC Systems Multi-Column/Continuous

Double the capacity, half the cost

Our multi-column chromatography systems offer significant advantages to those who need to increase capacity (de-bottleneck downstream) or reduce Protein A cost. Our Contichrom TWIN “2C-PCC” solution is built on YMC ChromaCon intelligence and patents and helps you take advantage of the most simple GMP scale multi-column technology.

When compared to conventional batch processes, the system delivers enhanced resin capacity utilization (up to 3X), decreased buffer consumption (by over 50% in many cases), and increased product concentration, all in a simple, two-column design that eases manufacturing validation and minimizes the risk of downtime while reducing operating expense.

Productivity and simplicity:

  • Using just two columns provides users with the lowest risk and simplest path to validation/qualification for regulated processes
  • Increased productivity: The Contichrom TWIN LPLC running the CaptureSMB process delivers increased productivity (up to threefold) while both reducing resin costs and decreasing buffer consumption by up to 50% (or more)
  • The risk of downtime and capital costs are minimized and operating expenses reduced when compared to other multi-column systems

Process intensification and flexibility on a single skid:

  • YMC’s Contichrom TWIN maximizes asset utilization and saves space and cost using pre-packed or conventional columns with options to have multiple modes of operation on a single skid
  • Multi-column continuous or extended batch operation
  • Conventional batch
  • Sequential polishing (two different steps (e.g., bind/elute – flow-through) with no holdup
  • Buffer In-line Dilution capability integrated

Proven scale-up, scale-down

  • GMP customers have proven the direct scale-up/scale-down with our Contichrom CUBE lab/bench instrument. The fluidic design of the Contichrom TWIN LPLC system allows for direct scale-up or scale-down to the Contichrom CUBE FPLC from YMC. Methods developed on the CUBE can be easily transferred to the Contichrom TWIN LPLC.
  • Intuitive software for twin-column operation: The Contichrom TWIN LPLC automation and control software is designed to be user-friendly. Operators can easily navigate and manage process setup, execution, and reporting. The software is designed to enable 21 CFR Part 11 compliance.
  • Advanced Pumps and Fluidics: The Contichrom TWIN LPLC system uses industry-leading LEWA ecodos pumps and is enhanced with an exclusive digital pump control technology providing (1) wide flow-range accuracy for improved product yield and purity and (2) industry-leading precision and batch-to-batch reproducibility.

To guarantee consistent and reproducible performance, the Contichrom TWIN LPLC system is designed with minimal holdup volumes and symmetric flow paths between the two columns, which was accomplished by optimal piping and components arrangements and sizing.

Simple, elegant, and includes:

  • Pump(s) with a hygienic design capable of delivering industry best-in-class precise flow
  • Multiple inlet and outlet valves
  • Air sensors to protect the columns or prevent dry running conditions
  • pH, conductivity, pressure, and temperature probes/sensors
  • Coreolis flow meters to control and/or verify pump flow rates
  • Dual wavelength UV monitors in the range of 280 and 300 nm
  • All necessary tubing, valves, instruments, connections, and safety devices for GMP process requirements
  • System automation and control with an easy-to-follow user interface

Application Search

Use our extensive Applications Search page to mine a dynamically growing collection of over 350 YMC-produced application notes.

The data can be searched by any combination of several variables, including sample classification, compound name (including partial names), and column parameters.

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