Phenyl columns provide unique selectivity when compared to aliphatic straight chain reversed-phase materials such as C18, C8, and C4. The π electrons of the phenyl group can interact with the aromatic ring(s) of an analyte to increase retention relative to non-aromatic species. Phenyl also has application as an alternative to C18 and C4 for the analysis of peptides and proteins on both small-pore (120Å) and wide-pore (300Å) silica supports. Retention is decreased on wide-pore phenyl phases relative to 120Å material. Generally, as pore size increases, surface area decreases, resulting in shorter retention times.
YMC-Pack Ph produces different separation characteristics from alkyl-silica stationary phases (C18, C8, C4) for analytes with aromatic rings due to the π – π and hydrophobic interactions contributing to the separation.
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