Preparative Spherical Packing Materials
YMC offers “bare” silica, silica-based normal phase, and a wide variety of silica-based reversed phase materials for preparative applications. These materials are available in bulk supply or packed in preparative scale columns. Particle sizes include: 10µm, 15µm, 20µm, 50µm, 75µm, and 150µm.
The particle size, pore size, and overall quality of all sizes of products are strictly controlled. All materials are available on conventional standard spherical silica, or on superior mechanical strength silica (YMC-HG type) for enhanced durability across unpacking/repacking cycles.
- Extensive variety of particle sizes, pore sizes, and separation chemistries.
- Specialized Reversed Phase chemistries for polar compounds, aromatics, biomolecules, aqueous eluents.
- Narrow distribution of particle size and pore size for better separations.
- Minimal occurrence of fines and fractured particles.
- High mechanical strength (HG-type) silica extends the durability of material that is unpacked and repacked.
- Suitable for preparative isolation using YMC pressure resistant glass or stainless steel columns.
Comparison of Mechanical Strength
Different materials have different levels of physical resiliency under the rigors of wetting with organic solvents and aqueous buffers. In addition, the physical stresses of un-packing and re-packing a column can lead to shearing and crushing of the packing material. The fractured particles – fines – congest the interstitial spacing of the spherical particles, which leads to an increase in backpressure.
The test results below compare the effect of packing/un-packing cycles on YMC conventional silica, YMC-HG type silica, and YMC-Triart hybrid silica. For applications where un-packing and re-packing are required, either YMC-HG-type (described on this page) or YMC-Triart (described elsewhere) materials demonstrate an advantage.
YMC Stationary Phase for Preparative Chromatography
(silica unless noted)
|PREP PARTICLE SIZES
|Triart C18||TA||Multilayered hybrid particle, conventional C18 hydrophobicity, though slightly more retentive, suitable for 100% aqueous mobile phase||120||5, 10, 15, 20||20||2 – 10 base washable at pH 12|
|Triart C8||TO||Multilayered hybrid particle, less hydrophobic than C8||200||5, 10, 15, 20||13||2 – 20 base washable at pH 12|
|Triart Phenyl||TPH||Multilayered hybrid particle, unique selectivity, suitable for aromatic compounds||120||5, 10, 15, 20||17||1 – 10|
|Triart PFP||TPF||Multilayered hybrid particle, unique selectivity, suitable for structural isomers, not endcapped||120||5, 10, 15, 20||15||1 – 8|
|Triart Diol HILIC||TDH||Multilayered hybrid particle, HILIC||120||5, 10, 15, 20||–||2 – 10|
|ProC18||AS||perfect endcapping to minimize the effect of residual silanols. ProC18 sets new standards for all types of organic molecules||120||10||16||2-8|
|ODS-A||AA||one of YMC’s international bestsellers, traditional high performance C18 column||120||10,15,20,50,75,150||17||2-7.5|
|ODS-AQ||AQ||hydrophilic C18 surface, metabolite recognition for aqueous eluent systems, substanitially increased retention of polar compounds||120||10,15,20,50||14||2-7.5|
|Polymer C18||PC||a high efficiency C18 on a polymeric support(no silanols) that is totally inert and enhances selectivity for aromatics, pH2-13||–||10||C18=10%||2-13|
|C8(Octyl)||OC||C8 fully endcapped, high coverage monomeric bonding chemistry||120||10,15,20||10||2-7.5|
|YMCbasic||BA||popular product with large customer base in the US, complementary selectivity pattern for basic compounds||proprietary||10||7||2-7.5|
|Phenyl||PH||monomeric phenyl bonding, endcapped||120||10,15,20||9||2-7.5|
|C4(Butyl)||BU||C4 fully endcapped, high coverage monomeric bonding chemistry||120||10,15,20||7||2-7.5|
|C1(TMS)||TM||trimethyl silane, excellent hydrolytic stability||120||10,15,20||4||2-7.5|
|Amino||NH||primary amino derivative, high coverage monomeric bonding chemistry||120||10||3||2-7.5|
|Cyano||CN||fully endcapped, useful also for SFC applications||120||10,15,20||7||2-7.5|
|Diol||DN||for normal phase applications, good selectivity without excessive retention||120||10,15||–||2-7.5|
|YMC Chiral Prep CD ST||ST||cyclodexstrin based stationary phase for chiral compounds||120||10,20 50||–||2-7|
|YMC Chiral Prep CD PM||PM||phenyl modified cyclodextrin based phase with different selectivity compared to ST||120||10,20 50||–||2-7|
|Silica||SL||ultra high purity, high mechanical stability||60||10,15,75,150||–||2-7.5|
|PROTEIN-RP||PR||This is a silica-based reversed-phase material designed for separation of proteins or peptides. It has strong acid resistance.||200||15||4||1.5-7.5|