Most Polar Reversed-Phase Column
In reversed-phase mode, cyano columns are the most polar and least retentive of all reversed-phase packings. Extremely hydrophobic compounds which do not elute in standard C18 and C8 packings can be retained using typical reversed-phase eluents. In addition, the unique nitrile chemistry provides different selectivity from both phenyl and standard aliphatic (C18, C8, and C4) reversed-phase packings. Large pore-size 300Å packing materials are also available for the analysis of biomolecules in order to utilize this unique selectivity.